New Respiratory Enterovirus and Recombinant Rhinoviruses among Circulating Picornaviruses

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Tapparel, Caroline ; Junier, Thomas ; Gerlach, Daniel ; Van Belle, Sandra ; Turin, Lara ; Cordey, Samuel ; Mühlemann, Kathrin ; Regamey, Nicolas ; Aubert, John-David ; Soccal, Paola M. ; Eigenmann, Philippe ; Zdobnov, Evgeny ; Kaiser, Laurent
• Description:
  Rhinoviruses and enteroviruses are leading causes of respiratory infections. To evaluate genotypic diversity and identify forces shaping picornavirus evolution, we screened persons with respiratory illnesses by using rhinovirus-specific or generic real-time PCR assays. We then sequenced the 5 untranslated region, capsid protein VP1, and protease precursor 3CD regions of virus-positive samples. Subsequent phylogenetic analysis identified the large genotypic diversity of rhinoviruses circulating in humans. We identified and completed the genome sequence of a new enterovirus genotype associated with respiratory symptoms and acute otitis media, confirming the close relationship between rhinoviruses and enteroviruses and the need to detect both viruses in respiratory specimens. Finally, we identified recombinants among circulating rhinoviruses and mapped their recombination sites, thereby demonstrating that rhinoviruses can recombine in their natural host. This study clarifies the diversity and explains the reasons for evolution of these viruses.
• Subjects:
  Adolescent Adult Capsid Protein Child Child, Preschool Enterovirus Enterovirus Infections Genetic Variation Genotype Humans Infant Infant, Newborn Molecular Epidemiology Nonstructural Protein Otitis Media Phylogeny Picornaviridae Infections Picornavirus Recombination Recombination, Genetic Research Respiratory Infections Respiratory Tract Infections Rhinovirus Sequence Analysis, DNA Young Adult

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• Source:
  Emerg Infect Dis. 15(5):719-726.
• Document Type:
  Journal Article
• Volume:
  15
• Issue:
  5
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:29461a0ac89e0c118ddbc393ac5b9a899443a90c3b9fbeebc1e26cdc3998f9ad
• Download URL:
  https://stacks.cdc.gov/view/cdc/17746/cdc_17746_DS1.pdf
• File Type:
  [PDF - 295.13 KB ]