Shiga Toxin–producing Escherichia coli Strains Negative for Locus of Enterocyte Effacement

Newton, Hayley J.; Sloan, Joan; Bulach, Dieter M.; Seemann, Torsten; Allison, Cody C.; Tauschek, Marija; Robins-Browne, Roy M.; Paton, James C.; Whittam, Thomas S.; Paton, Adrienne W.; Hartland, Elizabeth L.

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Shiga Toxin–producing Escherichia coli Strains Negative for Locus of Enterocyte Effacement

Supporting Files Public Domain

Mar 2009
By Newton, Hayley J. ; Sloan, Joan ; Bulach, Dieter M. ; ...

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Newton, Hayley J. ; Sloan, Joan ; Bulach, Dieter M. ; Seemann, Torsten ; Allison, Cody C. ; Tauschek, Marija ; Robins-Browne, Roy M. ; Paton, James C. ; Whittam, Thomas S. ; Paton, Adrienne W. ; Hartland, Elizabeth L.
Description:

Most Shiga toxin-producing Escherichia coli (STEC) infections that are associated with severe sequelae such as hemolytic uremic syndrome (HUS) are caused by attaching and effacing pathogens that carry the locus of enterocyte effacement (LEE). However, a proportion of STEC isolates that do not carry LEE have been associated with HUS. To clarify the emergence of LEE-negative STEC, we compared the genetic composition of the virulence plasmids pO113 and pO157 from LEE-negative and LEE-positive STEC, respectively. The complete nucleotide sequence of pO113 showed that several plasmid genes were shared by STEC O157:H7. In addition, allelic profiling of the ehxA gene demonstrated that pO113 belongs to a different evolutionary lineage than pO157 and that the virulence plasmids of LEE-negative STEC strains were highly related. In contrast, multilocus sequence typing of 17 LEE-negative STEC isolates showed several clonal groups, suggesting that pathogenic LEE-negative STEC has emerged several times throughout its evolution.
Subjects:

Bacterial Typing Techniques DNA Helicases EhxA Protein Escherichia Coli O157 Escherichia Coli Plasmids Escherichia Coli Proteins Foodborne Pathogens Hemolysin Proteins Hemolytic-Uremic Syndrome Humans Molecular Evolution Phosphoproteins Phylogeny Plasmids Research Sequence Analysis, DNA Shiga-Toxigenic Escherichia Coli STEC Trans-Activators Virulence
Source:

Emerg Infect Dis. 15(3):372-380.
Document Type:

Journal Article
Volume:

15
Issue:

3
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:fba6a60c07de8a0689ab24f0177f6ef3ce6f49dafe8c8b506e42c6c055a8a02e
Download URL:

https://stacks.cdc.gov/view/cdc/17681/cdc_17681_DS1.pdf
File Type:

[PDF - 316.92 KB ]

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