A whole transcriptomic approach provides novel insights into the molecular basis of organophosphate and pyrethroid resistance in Anopheles arabiensis from Ethiopia

Details

• Alternative Title:
  Insect Biochem Mol Biol
• Personal Author:
  Messenger, Louisa A. ; Impoinvil, Lucy Mackenzie ; Derilus, Dieunel ; Yewhalaw, Delenasaw ; Irish, Seth ; Lenhart, Audrey
• Description:
  The development of insecticide resistance in malaria vectors is of increasing concern in Ethiopia because of its potential implications for vector control failure. To better elucidate the specificity of resistance mechanisms and to facilitate the design of control strategies that minimize the likelihood of selecting for cross-resistance, a whole transcriptomic approach was used to explore gene expression patterns in a multi-insecticide resistant population of Anopheles arabiensis from Oromia Region, Ethiopia. This field population was resistant to the diagnostic doses of malathion (average mortality of 71.9%) and permethrin (77.4%), with pools of survivors and unexposed individuals analyzed using Illumina RNA-sequencing, alongside insecticide susceptible reference strains. This population also demonstrated deltamethrin resistance but complete susceptibility to alpha-cypermethrin, bendiocarb and propoxur, providing a phenotypic basis for detecting insecticide-specific resistance mechanisms. Transcriptomic data revealed overexpression of genes including cytochrome P450s, glutathione-s-transferases and carboxylesterases (including CYP4C36, CYP6AA1, CYP6M2, CYP6M3, CYP6P4, CYP9K1, CYP9L1, GSTD3, GSTE2, GSTE3, GSTE4, GSTE5, GSTE7 and two carboxylesterases) that were shared between malathion and permethrin survivors. We also identified nineteen highly overexpressed cuticular-associated proteins (including CYP4G16, CYP4G17 and chitinase) and eighteen salivary gland proteins (including D7r4 short form salivary protein), which may be contributing to a non-specific resistance phenotype by either enhancing the cuticular barrier or promoting binding and sequestration of insecticides, respectively. These findings provide novel insights into the molecular basis of insecticide resistance in this lesser well-characterized major malaria vector species.
• Subjects:
  Animals Anopheles Ethiopia Female Insecticide Resistance Mosquito Vectors Organophosphates Pyrethrins Transcriptome
• Keywords:
  Anopheles Arabiensis Insecticide Resistance Organophosphate Pyrethroid RNA-Seq
• Source:
  Insect Biochem Mol Biol. 139:103655
• Pubmed ID:
  34562591
• Pubmed Central ID:
  PMC11705372
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  139
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:368fc40cc70ddaa34b18770c45bbd95b6ee49fa6beaa20ee43275d3748b0fabd28b334f530eed6dedcca6f5c2ae17bdc0413341e8d8c631ace19ebb8b978a475
• Download URL:
  https://stacks.cdc.gov/view/cdc/175431/cdc_175431_DS1.pdf
• File Type:
  [PDF - 1.58 MB ]