Reproducibility of Serologic Assays for Influenza Virus A (H5N1)

Stephenson, Iain; Heath, Alan; Major, Diane; Newman, Robert W.; Hoschler, Katja; Junzi, Wang; Katz, Jacqueline M.; Weir, Jerry P.; Zambon, Maria C.; Wood, John M.

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Reproducibility of Serologic Assays for Influenza Virus A (H5N1)

Supporting Files Public Domain

Aug 2009
By Stephenson, Iain ; Heath, Alan ; Major, Diane ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Stephenson, Iain ; Heath, Alan ; Major, Diane ; Newman, Robert W. ; Hoschler, Katja ; Junzi, Wang ; Katz, Jacqueline M. ; Weir, Jerry P. ; Zambon, Maria C. ; Wood, John M.
Description:

Hemagglutination-inhibition (HI) and neutralization are used to evaluate vaccines against influenza virus A (H5N1); however, poor standardization leads to interlaboratory variation of results. A candidate antibody standard (07/150) was prepared from pooled plasma of persons given clade 1 A/Vietnam/1194/2004 vaccine. To test human and sheep antiserum, 15 laboratories used HI and neutralization and reassortant A/Vietnam/1194/2004, A/turkey/Turkey/1/2005 (clade 2.2), and A/Anhui/1/2005 (clade 2.3.4) viruses. Interlaboratory variation was observed for both assays, but when titers were expressed relative to 07/150, overall percentage geometric coefficient of variation for A/Vietnam/1194/2004 was reduced from 125% to 61% for HI and from 183% to 81% for neutralization. Lack of reduced variability to clade 2 antigens suggested the need for clade-specific standards. Sheep antiserum as a standard did not reliably reduce variability. The World Health Organization has established 07/150 as an international standard for antibody to clade 1 subtype H5 and has an assigned potency of 1,000 IU/ampoule.
Subjects:

Animals Antibodies, Viral Communicable Diseases, Emerging False Positive Reactions Hemagglutination Inhibition Tests Humans Influenza, Human Influenza A Virus, H5N1 Subtype Influenza Vaccines Laboratories Neutralization Tests Reference Standards Reproducibility Of Results Serologic Tests Sheep
Source:

Emerg Infect Dis. 15(8):1250-1259.
Document Type:

Journal Article
Name as Subject:

World Health Organization ; World Health Organization
Volume:

15
Issue:

8
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:5f2fb930f732ca43871eadb60e9de9182d2501b3e3a582ca606ed1ee48be15b7
Download URL:

https://stacks.cdc.gov/view/cdc/17502/cdc_17502_DS1.pdf
File Type:

[PDF - 580.30 KB ]

File Language:

English

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