i
Reproducibility of Serologic Assays for Influenza Virus A (H5N1)
-
Aug 2009
Source: Emerg Infect Dis. 15(8):1250-1259.
Details:
-
Alternative Title:Emerg Infect Dis
-
Personal Author:
-
Description:Hemagglutination-inhibition (HI) and neutralization are used to evaluate vaccines against influenza virus A (H5N1); however, poor standardization leads to interlaboratory variation of results. A candidate antibody standard (07/150) was prepared from pooled plasma of persons given clade 1 A/Vietnam/1194/2004 vaccine. To test human and sheep antiserum, 15 laboratories used HI and neutralization and reassortant A/Vietnam/1194/2004, A/turkey/Turkey/1/2005 (clade 2.2), and A/Anhui/1/2005 (clade 2.3.4) viruses. Interlaboratory variation was observed for both assays, but when titers were expressed relative to 07/150, overall percentage geometric coefficient of variation for A/Vietnam/1194/2004 was reduced from 125% to 61% for HI and from 183% to 81% for neutralization. Lack of reduced variability to clade 2 antigens suggested the need for clade-specific standards. Sheep antiserum as a standard did not reliably reduce variability. The World Health Organization has established 07/150 as an international standard for antibody to clade 1 subtype H5 and has an assigned potency of 1,000 IU/ampoule.
-
Subject:
-
Document Type:
-
Name as Subject:
-
Collection(s):
-
Main Document Checksum:
-
File Type:
Supporting Files
-
pdf txt txt gif jpeg gif jpeg gif jpeg pdf pdf
More +