Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Stephenson, Iain ; Heath, Alan ; Major, Diane ; Newman, Robert W. ; Hoschler, Katja ; ... More +
Stephenson, Iain ; Heath, Alan ; Major, Diane ; Newman, Robert W. ; Hoschler, Katja ; Junzi, Wang ; Katz, Jacqueline M. ; Weir, Jerry P. ; Zambon, Maria C. ; Wood, John M. Less -
Description:
Hemagglutination-inhibition (HI) and neutralization are used to evaluate vaccines against influenza virus A (H5N1); however, poor standardization leads to interlaboratory variation of results. A candidate antibody standard (07/150) was prepared from pooled plasma of persons given clade 1 A/Vietnam/1194/2004 vaccine. To test human and sheep antiserum, 15 laboratories used HI and neutralization and reassortant A/Vietnam/1194/2004, A/turkey/Turkey/1/2005 (clade 2.2), and A/Anhui/1/2005 (clade 2.3.4) viruses. Interlaboratory variation was observed for both assays, but when titers were expressed relative to 07/150, overall percentage geometric coefficient of variation for A/Vietnam/1194/2004 was reduced from 125% to 61% for HI and from 183% to 81% for neutralization. Lack of reduced variability to clade 2 antigens suggested the need for clade-specific standards. Sheep antiserum as a standard did not reliably reduce variability. The World Health Organization has established 07/150 as an international standard for antibody to clade 1 subtype H5 and has an assigned potency of 1,000 IU/ampoule.
Subject:
[+]
Animals
Antibodies, Viral
Communicable Diseases, Emerging
False Positive Reactions
Hemagglutination Inhibition Tests
Humans
Influenza, Human
Influenza A Virus, H5N1 Subtype
Influenza Vaccines
Laboratories
Neutralization Tests
Reference Standards
Reproducibility Of Results
Serologic Tests
Sheep

Source:
Emerg Infect Dis. 15(8):1250-1259.
Document Type:
Journal Article
Name as Subject:
World Health Organization
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:5f2fb930f732ca43871eadb60e9de9182d2501b3e3a582ca606ed1ee48be15b7
File Type:

Supporting Files

• 	08-1754_appT3-s3.pdf	pdf
  	08-1754_finalR.nxml	txt
  	license.txt	txt
  	08-1754-F1.gif	gif
  	08-1754-F1.jpg	jpeg
  	08-1754-F2.gif	gif
  	08-1754-F2.jpg	jpeg
  	08-1754-F3.gif	gif
  	08-1754-F3.jpg	jpeg
  	08-1754_appT1-s1.pdf	pdf
  	08-1754_appT2-s2.pdf	pdf

More +