Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Currie, Bart J. ; Haslem, Asha ; Pearson, Talima ; Hornstra, Heidie ; Leadem, Benjamin ; Mayo, Mark ; Gal, Daniel ; Ward, Linda ; Godoy, Daniel ; Spratt, Brian G. ; Keim, Paul
• Description:
  Endemic melioidosis is caused by genetically diverse Burkholderia pseudomallei strains. However, clonal outbreaks (multiple cases caused by 1 strain) have occurred, such as from contaminated potable water. B. pseudomallei is designated a group B bioterrorism agent, which necessitates rapidly recognizing point-source outbreaks. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) can identify genetically related isolates, but results take several days to obtain. We developed a simplified 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for rapid typing and compared results with PFGE and MLST for a large number of well-characterized B. pseudomallei isolates. MLVA-4 compared favorably with MLST and PFGE for the same isolates; it discriminated between 65 multilocus sequence types and showed relatedness between epidemiologically linked isolates from outbreak clusters and between isolates from individual patients. MLVA-4 can establish or refute that a clonal outbreak of melioidosis has occurred within 8 hours of receipt of bacterial strains.
• Subjects:
  Bacterial Typing Techniques Base Sequence Disease Outbreaks Electrophoresis, Gel, Pulsed-Field Humans Melioidosis Minisatellite Repeats Molecular Sequence Data Sequence Analysis, DNA
• Source:
  Emerg Infect Dis. 15(2):169-174.
• Document Type:
  Journal Article
• Volume:
  15
• Issue:
  2
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:889a756fdbf89de75c250d7283759349e10b4c721c994268323295f646b4bfd8
• Download URL:
  https://stacks.cdc.gov/view/cdc/17415/cdc_17415_DS1.pdf
• File Type:
  [PDF - 237.07 KB ]