Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Dubois, Melissa E. ; Slifka, Mark K.
Dubois, Melissa E. ; Slifka, Mark K. Less -
Description:
Serologic cross-reactivity between orthopoxviruses is a substantial barrier to laboratory diagnosis of specific orthopoxvirus infections and epidemiologic characterization of disease outbreaks. Historically, time-consuming and labor-intensive strategies such as cross-adsorbed neutralization assays, immunofluorescence assays, and hemagglutination-inhibition assays have been used to identify orthopoxvirus infections. We used cross-adsorption to develop a simple and quantitative postadsorption ELISA for distinguishing between monkeypox and vaccinia infections. Despite the difficulty of diagnosing clinically inapparent monkeypox in previously vaccinated persons, this technique exhibited 100% sensitivity and 100% specificity for identifying clinically overt monkeypox infection irrespective of vaccination history. We also describe a Western blot technique in which up to 3 diagnostic bands may be used to distinguish between vaccinia and monkeypox infection. The techniques described provide independent diagnostic tests suitable for retrospective analysis of monkeypox outbreaks.
Subject:
[+]
Adult
Antibodies, Viral
Blotting, Western
Cell Line
Differential Diagnosis
Disease Outbreaks
Enzyme-Linked Immunosorbent Assay
Humans
Monkeypox
Monkeypox Virus
Research
Retrospective Studies
Sensitivity And Specificity
Serologic Diagnostics
Vaccinia
Wisconsin

Source:
Emerg Infect Dis. 14(4):592-599.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:1796bdfac0d60bc7d568d1d63375d8b8acb94541ca7397f7c16761c48452a8a1
File Type:

Supporting Files

• 	license.txt	txt
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  	07-1044-F1.jpg	jpeg
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  	07-1044-F3.jpg	jpeg
  	07-1044_finalR.nxml	txt
  	07-1044_Techapp.pdf	pdf

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