Retrospective Analysis of Monkeypox Infection

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Dubois, Melissa E. ; Slifka, Mark K.
• Description:
  Serologic cross-reactivity between orthopoxviruses is a substantial barrier to laboratory diagnosis of specific orthopoxvirus infections and epidemiologic characterization of disease outbreaks. Historically, time-consuming and labor-intensive strategies such as cross-adsorbed neutralization assays, immunofluorescence assays, and hemagglutination-inhibition assays have been used to identify orthopoxvirus infections. We used cross-adsorption to develop a simple and quantitative postadsorption ELISA for distinguishing between monkeypox and vaccinia infections. Despite the difficulty of diagnosing clinically inapparent monkeypox in previously vaccinated persons, this technique exhibited 100% sensitivity and 100% specificity for identifying clinically overt monkeypox infection irrespective of vaccination history. We also describe a Western blot technique in which up to 3 diagnostic bands may be used to distinguish between vaccinia and monkeypox infection. The techniques described provide independent diagnostic tests suitable for retrospective analysis of monkeypox outbreaks.
• Subjects:
  Adult Antibodies, Viral Blotting, Western Cell Line Disease Outbreaks Enzyme-Linked Immunosorbent Assay Humans Monkeypox Virus Mpox (Mpox (monkeypox)) Retrospective Studies
• Source:
  Emerg Infect Dis. 14(4):592-599.
• Document Type:
  Journal Article
• Volume:
  14
• Issue:
  4
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:1796bdfac0d60bc7d568d1d63375d8b8acb94541ca7397f7c16761c48452a8a1
• Download URL:
  https://stacks.cdc.gov/view/cdc/16988/cdc_16988_DS1.pdf
• File Type:
  [PDF - 389.92 KB ]