Genetic Diversity among Clonal Lineages within Escherichia coli O157:H7 Stepwise Evolutionary Model

Feng, Peter C.H.; Monday, Steven R.; Lacher, David W.; Allison, Lesley; Siitonen, Anja; Keys, Christine; Eklund, Marjut; Nagano, Hideki; Karch, Helge; Keen, James; Whittam, Thomas S.

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Genetic Diversity among Clonal Lineages within Escherichia coli O157:H7 Stepwise Evolutionary Model

Supporting Files Public Domain

Nov 2007
By Feng, Peter C.H. ; Monday, Steven R. ; Lacher, David W. ; ...

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Alternative Title:

Emerg Infect Dis
Personal Author:

Feng, Peter C.H. ; Monday, Steven R. ; Lacher, David W. ; Allison, Lesley ; Siitonen, Anja ; Keys, Christine ; Eklund, Marjut ; Nagano, Hideki ; Karch, Helge ; Keen, James ; Whittam, Thomas S.
Description:

Escherichia coli O157:H7 variants were examined for trait mutations and by molecular subtyping to better define clonal complexes postulated on the O157:H7 evolution model. Strains of beta-glucuronidase-positive, sorbitol-negative O157:H7 isolated in United States and Japan were identical to A5 clonal strain and shared sequence type (ST)-65 by multilocus sequence typing (MLST); thus, they belong in A5. However, these strains exhibited pulsed-field gel electrophoresis (PFGE) profile differences that suggested genomic divergence between populations. Sorbitol-fermenting O157 (SFO157) strains from Finland, Scotland, and Germany were identical to A4 clonal strain and belong in A4. Some SFO157 strains, isolated years apart and from different countries, had identical PFGE profiles, suggesting a common origin. Despite similarities, some Finnish and Scottish and all of the German strains have ST-75 ("German clone"), whereas others have ST-76, a new variant ("Scottish clone"). MLST of strains in other clonal complexes also discriminated strains thought to be identical and showed that genetic differences will further distinguish clonal populations into subclones.
Subjects:

Clonal Complexes Electrophoresis, Gel, Pulsed-Field Enterohemorrhagic E. Coli O157:H7 Escherichia Coli Infections Escherichia Coli O157 Evolution, Molecular Genetic Diversity Genetic Variation Humans Molecular Evolution Research
Source:

Emerg Infect Dis. 13(11):1701-1706.
Document Type:

Journal Article
Volume:

13
Issue:

11
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:38330f5365dcfa381e4dd400cc051a19f212133ef90426ec86bca9c11ab8e159
Download URL:

https://stacks.cdc.gov/view/cdc/16785/cdc_16785_DS1.pdf
File Type:

[PDF - 333.51 KB ]

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