Genetic Diversity of Bartonella henselae in Human Infection Detected with Multispacer Typing

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Li, Wenjun ; Raoult, Didier ; Fournier, Pierre-Edouard
• Description:
  We applied multispacer typing (MST) by incorporating 9 variable intergenic spacers to Bartonella henselae DNA detected in lymph node biopsy specimens from 70 patients with cat-scratch disease (CSD), in cardiac valve specimens from 2 patients with endocarditis, and in 3 human isolates from patients with bacillary angiomatosis, CSD, and endocarditis. Sixteen MST genotypes were found, 5 previously identified in cats and 11 new. Of the studied DNA, 78.7% belonged to 2 genotypes, which were phylogenetically organized into 4 lineages. Human strains were mostly grouped within 2 lineages, previously identified as Marseille and Houston-1. Our results suggest a greater genetic diversity in human-infecting B. henselae than what has previously been evaluated by using other genotyping methods. However, the diversity is not significantly different from that of cat strains. MST is thus a suitable genotyping tool for evaluating the genetic heterogeneity of B. henselae among isolates obtained from human patients.
• Subjects:
  Animals Bacterial Typing Techniques Bartonella Henselae Base Sequence Cat-Scratch Disease Cats DNA, Bacterial DNA, Intergenic Genetic Variation Humans Polymerase Chain Reaction

  More +
• Source:
  Emerg Infect Dis. 13(8):1178-1183.
• Document Type:
  Journal Article
• Volume:
  13
• Issue:
  8
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:9398631e62c5e90c0943d5b7be4d5af87bf01cbda5af37b8c2bb9d69028acae5
• Download URL:
  https://stacks.cdc.gov/view/cdc/16696/cdc_16696_DS1.pdf
• File Type:
  [PDF - 186.74 KB ]