A Serological Multiplexed Immunoassay (MIA) Detects Antibody Reactivity to SARS-CoV-2 and Other Viral Pathogens in Liberia and Is Configurable as a Multiplexed Inhibition Test (MINT)

Details

• Alternative Title:
  Immuno
• Personal Author:
  Haun, Brien K. ; To, Albert ; Williams, Caitlin A. ; Ball, Aquena ; Fong, Karalyn ; Wong, Teri Ann S. ; Shobayo, Bode ; Teahton, Julius ; Ching, Lauren ; Kamara, Varney ; Tekah, Davidetta M. ; Humphrey, Peter ; Berestecky, John ; Nerurkar, Vivek R. ; Lehrer, Axel T.
• Description:
  The SARS-CoV-2 pandemic ignited global efforts to rapidly develop testing, therapeutics, and vaccines. However, the rewards of these efforts were slow to reach many low- to middle-income countries (LMIC) across the African continent and globally. Therefore, two bead-based multiplexed serological assays were developed to determine SARS-CoV-2 exposure across four counties in Liberia. This study was conducted during the summer of 2021 on 189 samples collected throughout Grand Bassa, Bong, Margibi, and Montserrado counties. Our multiplexed immunoassay (MIA) detected elevated exposure to SARS-CoV-2 and multiple variant antigens. Additionally, we detected evidence of exposure to Dengue virus serotype 2, Chikungunya virus, and the seasonal coronavirus NL63. Our multiplexed inhibition test (MINT) was developed from the MIA to observe antibody-mediated inhibition of SARS-CoV-2 spike protein binding to its cognate cellular receptor ACE-2. We detected inhibitory antibodies in the tested Liberian samples, which were collectively consistent with a convalescent serological profile. These complementary assays serve to supplement existing serological testing needs and may enhance the technical capacity of scientifically underrepresented regions globally.
• Keywords:
  Antibodies CHIKV DENV-2 HCoV-NL63 Inhibition Liberia SARS-CoV-2 Serological Surveillance West-Africa
• Source:
  Immuno. 4(1):108-124
• Pubmed ID:
  39391865
• Pubmed Central ID:
  PMC11465787
• Document Type:
  Journal Article
• Funding:
  D71 TW010434/TW/FIC NIH HHSUnited States/ ; P30 GM114737/GM/NIGMS NIH HHSUnited States/ ; R01 AI132323/AI/NIAID NIH HHSUnited States/ ; U01 GH002256/GH/CGH CDC HHSUnited States/
• Volume:
  4
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:7dd7cb506f14bd21baf560d2cbfa46fbfd836b85d46c0a951a486144489200ca97ea8b9386ee103b39b892a17155eb3c92c99e435da90763b35e22c562fbc657
• Download URL:
  https://stacks.cdc.gov/view/cdc/165787/cdc_165787_DS1.pdf
• File Type:
  [PDF - 1.38 MB ]