Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1
Published Date:Mar 2007
Source:Emerg Infect Dis. 13(3):426-435.
Amino Acid Sequence
Drug Evaluation, Preclinical
Influenza A Virus, H5N1 Subtype
Influenza A Virus H1N1 Subtype
Influenza A Virus H3N2 Subtype
Influenza A Virus H5N1 Subtype
Mice, Inbred BALB C
Molecular Sequence Data
Viral Matrix Proteins
Description:Changes in influenza viruses require regular reformulation of strain-specific influenza vaccines. Vaccines based on conserved antigens provide broader protection. Influenza matrix protein 2 (M2) is highly conserved across influenza A subtypes. To evaluate its efficacy as a vaccine candidate, we vaccinated mice with M2 peptide of a widely shared consensus sequence. This vaccination induced antibodies that cross-reacted with divergent M2 peptide from an H5N1 subtype. A DNA vaccine expressing full-length consensus-sequence M2 (M2-DNA) induced M2-specific antibody responses and protected against challenge with lethal influenza. Mice primed with M2-DNA and then boosted with recombinant adenovirus expressing M2 (M2-Ad) had enhanced antibody responses that crossreacted with human and avian M2 sequences and produced T-cell responses. This M2 prime-boost vaccination conferred broad protection against challenge with lethal influenza A, including an H5N1 strain. Vaccination with M2, with key sequences represented, may provide broad protection against influenza A.
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