Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Tompkins, Stephen Mark ; Zhao, Zi-Shan ; Lo, Chia-Yun ; Misplon, Julia A. ; Liu, Teresa ; Ye, Zhiping ; Hogan, Robert J. ; Wu, Zhengqi ; Benton, Kimberly A. ; Tumpey, Terrence M. ; Epstein, Suzanne L.
• Description:
  Changes in influenza viruses require regular reformulation of strain-specific influenza vaccines. Vaccines based on conserved antigens provide broader protection. Influenza matrix protein 2 (M2) is highly conserved across influenza A subtypes. To evaluate its efficacy as a vaccine candidate, we vaccinated mice with M2 peptide of a widely shared consensus sequence. This vaccination induced antibodies that cross-reacted with divergent M2 peptide from an H5N1 subtype. A DNA vaccine expressing full-length consensus-sequence M2 (M2-DNA) induced M2-specific antibody responses and protected against challenge with lethal influenza. Mice primed with M2-DNA and then boosted with recombinant adenovirus expressing M2 (M2-Ad) had enhanced antibody responses that crossreacted with human and avian M2 sequences and produced T-cell responses. This M2 prime-boost vaccination conferred broad protection against challenge with lethal influenza A, including an H5N1 strain. Vaccination with M2, with key sequences represented, may provide broad protection against influenza A.
• Subjects:
  Adenoviridae Amino Acid Sequence Animals Antibodies, Viral Cross Reactions DNA Vaccines Drug Evaluation, Preclinical Female Genes, Viral Genetic Vectors Immunization Schedule Influenza A Virus, H5N1 Subtype Influenza A Virus H1N1 Subtype Influenza A Virus H3N2 Subtype Influenza A Virus H5N1 Subtype Influenza Vaccines Injections, Intramuscular Injections, Intraperitoneal Ion Channels M2 Protein Mice Mice, Inbred BALB C Molecular Sequence Data Orthomyxoviridae Infections Recombinant Proteins Research Sequence Alignment T-Lymphocytes Vaccination Vaccines, DNA Vaccines, Synthetic Viral Matrix Proteins

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• Source:
  Emerg Infect Dis. 13(3):426-435.
• Document Type:
  Journal Article
• Volume:
  13
• Issue:
  3
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:f5eab70db0a73c96fb4b9bf2dfd3af503c6f1377fa4e7a219a5fa9e4418adb1b
• Download URL:
  https://stacks.cdc.gov/view/cdc/16535/cdc_16535_DS1.pdf
• File Type:
  [PDF - 430.68 KB ]