Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Palacios, Gustavo ; Quan, Phenix-Lan ; Jabado, Omar J. ; Conlan, Sean ; Hirschberg, David L. ; Liu, Yang ; Zhai, Junhui ; Renwick, Neil ; Hui, Jeffrey ; Hegyi, Hedi ; Grolla, Allen ; Strong, James E. ; Towner, Jonathan S. ; Geisbert, Thomas W. ; Jahrling, Peter B. ; Büchen-Osmond, Cornelia ; Ellerbrok, Heinz ; Sanchez-Seco, Maria Paz ; Lussier, Yves ; Formenty, Pierre ; Nichol, Stuart T. ; Feldmann, Heinz ; Briese, Thomas ; Lipkin, W. Ian
• Description:
  To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004-2005.
• Subjects:
  Communicable Diseases Disease Outbreaks Fatal Outcome Humans Malaria, Falciparum Oligonucleotide Array Sequence Analysis Phylogeny Research Sensitivity And Specificity Virus Diseases
• Source:
  Emerg Infect Dis. 13(1):73-81.
• Document Type:
  Journal Article
• Funding:
  AI056118/AI/NIAID NIH HHS/United States ; AI51292/AI/NIAID NIH HHS/United States ; AI55466/AI/NIAID NIH HHS/United States ; K22 LM008308/LM/NLM NIH HHS/United States ; K22 LM008308-04/LM/NLM NIH HHS/United States ; U01AI070411/AI/NIAID NIH HHS/United States ; U54AI57158/AI/NIAID NIH HHS/United States
• Volume:
  13
• Issue:
  1
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:a1e724df832d384ac9141752ce3532973ecbf8bfbffed91a54f7d1d5a6c64258
• Download URL:
  https://stacks.cdc.gov/view/cdc/16456/cdc_16456_DS1.pdf
• File Type:
  [PDF - 372.51 KB ]