Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Heikinheimo, Annamari ; Lindström, Miia ; Granum, Per Einar ; Korkeala, Hannu
Heikinheimo, Annamari ; Lindström, Miia ; Granum, Per Einar ; Korkeala, Hannu Less -
Description:
We found a prevalence of 18% for enterotoxin gene-carrying (cpe+) Clostridium perfringens in the feces of healthy food handlers by PCR and isolated the organism from 11 of 23 PCR-positive persons by using hydrophobic grid membrane filter-colony hybridization. Several different cpe genotypes were recovered. The prevalence was 3.7% for plasmidial IS1151-cpe, 2.9% for plasmidial IS1470-like-cpe, 0.7% for chromosomal IS1470-cpe, and 1.5% for unknown cpe genotype. Lateral spread of cpe between C. perfringens strains was evident because strains from the same person carried IS1470-like cpe but shared no genetic relatedness according to pulsed-field gel electrophoresis analysis. Our findings suggest that healthy humans serve as a rich reservoir for cpe+ C. perfringens type A and may play a role in the etiology of gastrointestinal diseases caused by this organism. The results also indicate that humans should be considered a risk factor for spread of C. perfringens type A food poisoning and that they are a possible source of contamination for C. perfringens type A food poisoning.
Subject:
[+]
Animals
Cercopithecus Aethiops
Chromosome
Clostridium Perfringens
Cpe
Electrophoresis, Gel, Pulsed-Field
Enterotoxins
Epidemiology
Feces
Female
Foodborne Diseases
Food Handling
Genotype
Humans
Male
Plasmid
Research
Type A
Vero Cells
Feces

Source:
Emerg Infect Dis. 12(11):1724-1729.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:981ff487deccba16652afddea93963183a99aa8a9b96db8862a7d601df3f28ec
File Type:

Supporting Files

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