Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Gerrish, Robert S. ; Lee, James E. ; Reed, June ; Williams, Joel ; Farrell, Larry D. ; ... More +
Gerrish, Robert S. ; Lee, James E. ; Reed, June ; Williams, Joel ; Farrell, Larry D. ; Spiegel, Kathleen M. ; Sheridan, Peter P. ; Shields, Malcolm S. Less -
Description:
We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%-20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2.
Subjects:
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Adhesins, Bacterial Blotting, Southern Dispatch Enterohemorrhagic Escherichia Coli Escherichia Coli Proteins Hemolysin Proteins Nucleic Acid Hybridization Polymerase Chain Reaction Shiga Toxin 1 Shiga Toxin 2 Virulence Factors
Source:
Emerg Infect Dis. 13(8):1253-1255.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:c0175b35875a0f46865945dc36cb254ae0bcd14b04f115492250e1348f43681c
Download URL:
https://stacks.cdc.gov/view/cdc/16318/cdc_16318_DS1.pdf
File Type:

Supporting Files

• 	06-0428_appT-s1.pdf	pdf
  	06-0428_finalD.nxml	txt
  	license.txt	txt

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