Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Gerrish, Robert S. ; Lee, James E. ; Reed, June ; Williams, Joel ; Farrell, Larry D. ; ... More +
Gerrish, Robert S. ; Lee, James E. ; Reed, June ; Williams, Joel ; Farrell, Larry D. ; Spiegel, Kathleen M. ; Sheridan, Peter P. ; Shields, Malcolm S. ; Less -
Description:
We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%-20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2.
Subject:
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Adhesins, Bacterial
Blotting, Southern
Dispatch
Enterohemorrhagic Escherichia Coli
Escherichia Coli Proteins
Hemolysin Proteins
Nucleic Acid Hybridization
Polymerase Chain Reaction
Shiga Toxin 1
Shiga Toxin 2
Virulence Factors

Source:
Emerg Infect Dis. 13(8):1253-1255. ;
Document Type:
Journal Article ;
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
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urn:sha256:c0175b35875a0f46865945dc36cb254ae0bcd14b04f115492250e1348f43681c
File Type:

Supporting Files

• 	06-0428_appT-s1.pdf	pdf
  	06-0428_finalD.nxml	txt
  	license.txt	txt

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