PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli

Gerrish, Robert S.; Lee, James E.; Reed, June; Williams, Joel; Farrell, Larry D.; Spiegel, Kathleen M.; Sheridan, Peter P.; Shields, Malcolm S.

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PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli

Published Date:

Aug 2007

Source:

Emerg Infect Dis. 13(8):1253-1255.

[PDF - 1.91 MB]

Details:

Personal Authors:

Gerrish, Robert S. ; Lee, James E. ; Reed, June ; Williams, Joel ; Farrell, Larry D. ; ... More ▼

Keywords:

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Description:

We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%-20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2.

Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases

Supporting Files:

06-0428_appT-s1.pdf	application/pdf
06-0428_finalD.nxml	text/plain
license.txt	text/plain

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