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PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli
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Aug 2007
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Source: Emerg Infect Dis. 13(8):1253-1255.
[PDF-1.91 MB]
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Alternative Title:Emerg Infect Dis
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Description:We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%-20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2.
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Volume:13
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Issue:8
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