PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Gerrish, Robert S. ; Lee, James E. ; Reed, June ; Williams, Joel ; Farrell, Larry D. ; Spiegel, Kathleen M. ; Sheridan, Peter P. ; Shields, Malcolm S.
• Description:
  We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%-20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2.
• Subjects:
  Adhesins, Bacterial Blotting, Southern Dispatch Enterohemorrhagic Escherichia Coli Escherichia Coli Proteins Hemolysin Proteins Nucleic Acid Hybridization Polymerase Chain Reaction Shiga Toxin 1 Shiga Toxin 2 Virulence Factors

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• Source:
  Emerg Infect Dis. 13(8):1253-1255.
• Document Type:
  Journal Article
• Volume:
  13
• Issue:
  8
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:c0175b35875a0f46865945dc36cb254ae0bcd14b04f115492250e1348f43681c
• Download URL:
  https://stacks.cdc.gov/view/cdc/16318/cdc_16318_DS1.pdf
• File Type:
  [PDF - 1.91 MB ]