Diagnosis of Tuberculosis by an Enzyme-Linked Immunospot Assay for Interferon-γ

Wang, Jann-Yuan; Chou, Chien-Hong; Lee, Li-Na; Hsu, Hsiao-Leng; Jan, I-Shiow; Hsueh, Po-Ren; Yang, Pan-Chyr; Luh, Kwen-Tay

doi:10.3201/eid1304.051195

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Diagnosis of Tuberculosis by an Enzyme-Linked Immunospot Assay for Interferon-γ

Published Date:

Apr 2007

Publisher's site:

http://dx.doi.org/10.3201/eid1304.051195 New Site Icon

Source:

Emerg Infect Dis. 13(4):553-558.

[PDF-194.35 KB]

Details:

Alternative Title:

Emerg Infect Dis

Personal Author:

Wang, Jann-Yuan ; Chou, Chien-Hong ; Lee, Li-Na ; Hsu, Hsiao-Leng ; Jan, I-Shiow ; ... More ▼

Description:

We evaluated an enzyme-linked immunospot assay for interferon-gamma (T SPOT-TB) for rapid diagnosis of active tuberculosis (TB) in a disease-endemic area. From January to June 2005, patients whose clinical symptoms and radiographic findings were compatible with TB were recruited, and a blood sample was obtained for T SPOT-TB assay within 7 days of microbiologic studies. Sixty-five patients were studied, including 39 (60%) with active TB. Thirty-five (53.8%) patients had underlying medical conditions. Thirty-seven patients had positive cultures for Mycobacterium tuberculosis, and 11 patients had positive cultures for nontuberculous mycobacteria. The sensitivity, specificity, positive predictive value, and negative predictive value of the T SPOT-TB assay were 87.2%, 88.5%, 91.9%, and 82.1%, respectively. The accuracy of this test in diagnosing active TB is >80%, even in an area with a high incidence of nontuberculous mycobacterial disease.

Subject:

[+]

Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases

Main Document Checksum:

[+]

Supporting Files:

	05-1195.nxml	text/plain
	license.txt	text/plain

No Related Documents.

You May Also Like:

Updated guidelines for using interferon gamma release assays to detect Mycobacterium tuberculosis infection -- United States, 2010

Personal Author:

Castro, Kenneth G.; Goldberg, Stefan; Jereb, John A.; LoBue, Philip; Mazurek, Gerald H.; Vernon, Andrew;

Corporate Authors:

National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (U.S.), Division of Tuberculosis Elimination.. Centers for Disease Control and Prevention (U.S.). HIV Medicine Association. Infectious Diseases Society of America. National Institutes of Health (U.S.).

Published Date:

June 25, 2010

Series:

MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports ; v. 59, no. RR-5

Description:

"This report updates and combines earlier versions of guidelines for the prevention and treatment of opportunistic infections (OIs) in HIV-infected adults (i.e., persons aged [more than or equal to] 18 years) and adolescents (i.e., persons aged ...

Status:

current

File Type:

[PDF - 501.08 KB]
Risk for Tuberculosis among Children

Personal Author:

Nakaoka, Hiroshi; Lawson, Lovett; Squire, S. Bertel; Coulter, Brian; Ravn, Pernille; Brock, Inger; Hart, C. Anthony; Cuevas, Luis E.;

Published Date:

Sep 2006

Source:

Emerg Infect Dis. 12(9):1383-1388.

Description:

Contacts of adults with tuberculosis (TB) are at risk for infection. Tests based on interferon-gamma (IFN-gamma) expression in response to Mycobacterium tuberculosis antigens may be more sensitive than the tuberculin skin test (TST). Risk for infecti...

File Type:

[PDF - 127.35 KB]
Characteristics Associated with Negative Interferon-γ Release Assay Results in Culture-Confirmed Tuberculosis Patients, Texas, USA, 2013–2015

Personal Author:

Nguyen, Duc T.; Teeter, Larry D.; Graves, Julie; Graviss, Edward A.;

Published Date:

Mar 2018

Source:

Emerg Infect Dis. 24(3):534-540.

Description:

Interferon-γ release assays (IGRAs) are the preferred diagnostic test for tuberculosis (TB) infection in at-risk populations in developed countries. However, IGRAs have high false-negative rates in patients with TB disease. Population-based studies ...

File Type:

[PDF - 2.00 MB]
Unusual Interferon Gamma Measurements with QuantiFERON-TB Gold and QuantiFERON-TB Gold In-Tube Tests

Personal Author:

Powell, Richard D.; Whitworth, William C.; Bernardo, John; Moonan, Patrick K.; Mazurek, Gerald H.;

Published Date:

Jun 08 2011

Source:

PLoS One. 2011; 6(6).

Description:

IntroductionInterferon gamma (IFN-γ) release assays, such as QuantiFERON®-TB Gold test (QFT-G) and QuantiFERON®-TB Gold In-Tube test (QFT-GIT) are designed to detect M. tuberculosis (Mtb) infection. Recognition of unusual IFN-γ measurements may h...

File Type:

[PDF - 252.88 KB]
Multiple Cytokines Are Released When Blood from Patients with Tuberculosis Is Stimulated with Mycobacterium tuberculosis Antigens

Personal Author:

Kellar, Kathryn L.; Gehrke, Jennifer; Weis, Stephen E.; Mahmutovic-Mayhew, Aida; Davila, Blachy; Zajdowicz, Margan J.; Scarborough, Robin; LoBue, Philip A.; Lardizabal, Alfred A.; Daley, Charles L.; Reves, Randall R.; Bernardo, John; Campbell, Brandon H.; Whitworth, William C.; Mazurek, Gerald H.;

Published Date:

Nov 21 2011

Source:

PLoS One. 2011; 6(11).

Description:

BackgroundMycobacterium tuberculosis (Mtb) infection may cause overt disease or remain latent. Interferon gamma release assays (IGRAs) detect Mtb infection, both latent infection and infection manifesting as overt disease, by measuring whole-blood in...

File Type:

[PDF - 772.08 KB]