Diagnosis of Tuberculosis by an Enzyme-Linked Immunospot Assay for Interferon-γ

Wang, Jann-Yuan; Chou, Chien-Hong; Lee, Li-Na; Hsu, Hsiao-Leng; Jan, I-Shiow; Hsueh, Po-Ren; Yang, Pan-Chyr; Luh, Kwen-Tay

doi:10.3201/eid1304.051195

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Diagnosis of Tuberculosis by an Enzyme-Linked Immunospot Assay for Interferon-γ

Published Date:

Apr 2007

Publisher's site:

http://dx.doi.org/10.3201/eid1304.051195 New Site Icon

Source:

Emerg Infect Dis. 13(4):553-558.

[PDF - 194.35 KB]

Details:

Personal Authors:

Wang, Jann-Yuan ; Chou, Chien-Hong ; Lee, Li-Na ; Hsu, Hsiao-Leng ; Jan, I-Shiow ; ... More ▼

Keywords:

[+]

Description:

We evaluated an enzyme-linked immunospot assay for interferon-gamma (T SPOT-TB) for rapid diagnosis of active tuberculosis (TB) in a disease-endemic area. From January to June 2005, patients whose clinical symptoms and radiographic findings were compatible with TB were recruited, and a blood sample was obtained for T SPOT-TB assay within 7 days of microbiologic studies. Sixty-five patients were studied, including 39 (60%) with active TB. Thirty-five (53.8%) patients had underlying medical conditions. Thirty-seven patients had positive cultures for Mycobacterium tuberculosis, and 11 patients had positive cultures for nontuberculous mycobacteria. The sensitivity, specificity, positive predictive value, and negative predictive value of the T SPOT-TB assay were 87.2%, 88.5%, 91.9%, and 82.1%, respectively. The accuracy of this test in diagnosing active TB is >80%, even in an area with a high incidence of nontuberculous mycobacterial disease.

Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases

Supporting Files:

05-1195.nxml	text/plain
license.txt	text/plain

No Related Documents.

You May Also Like:

The Diagnosis of Human Fascioliasis by Enzyme-Linked Immunosorbent Assay (ELISA) Using Recombinant Cathepsin L Protease

Personal Author:

Gonzales Santana, Bibiana; Dalton, John P.; Vasquez Camargo, Fabio; Parkinson, Michael; Ndao, Momar;

Published:

Sep 19 2013

Source:

PLoS Negl Trop Dis. 2013; 7(9).

Description:

Background : Fascioliasis is a worldwide parasitic disease of domestic animals caused by helminths of the genus Fasciola. In many parts of the world, particularly in poor rural areas where animal disease is endemic, the parasite also infects humans. ...

File Type:

[PDF - 328.61 KB]
Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Dengue Capsid

Personal Author:

Selvarajah, Suganya; Chatterji, Udayan; Kuhn, Richard; Kinney, Richard; Vasudevan, Subhash G; Gallay, Philippe;

Published:

Mar 29 2012

Source:

Open Virol J. 2012; 6:29-37.

Description:

The astonishing speed with which Dengue has spread across the world and the severity of its infection make Dengue a prime threat to human life worldwide. Unfortunately, to date there are no effective vaccines or treatments against Dengue. Since only ...

File Type:

[PDF - 2.36 MB]
An Indirect Homologous Competitive Enzyme-linked Immunosorbent Assay for the Detection of a Class of Glycosylated Dihydrochalcones

Personal Author:

Ranganathan, Anupama; Paradise, Grace A.; Hansen, Chad A.; McCoy, Mark R.; Gee, Shirley J.; Zhong, Ping; Chang, Dan; Hammock, Bruce D.;

Published:

Jul 02 2013

Source:

J Agric Food Chem. 61(28):6964-6970.

Description:

Hesperetin dihydrochalcone 4'-glucoside, 1, and phloretin 4'-glucoside, 2, belong to a family of dihydrochalcone glycosides that exhibit flavorant properties. In this study was developed a competitive, indirect homologous ELISA for the dete...

File Type:

[PDF - 694.69 KB]
Detection by Enzyme-Linked Immunosorbent Assay of Antibodies to West Nile virus in Birds

Personal Author:

Ebel, Gregory D.; Dupuis, Alan P.; Nicholas, David; Young, Donna; Maffei, Joseph; Kramer, Laura D.;

Published:

Sep 2002

Source:

Emerg Infect Dis. 8(9):979-982.

Description:

We adapted an indirect immunoglobulin G enzyme-linked immunosorbent assay to facilitate studies of West Nile virus (WNV) and evaluated its application to taxonomically diverse avian species. Anti-WNV antibodies were detected in 23 bird species, inclu...

File Type:

[PDF - 434.42 KB]
Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide

Personal Author:

Hua, Xiude; Liu, Xiaofeng; Shi, Haiyan; Wang, Yanru; Kim, Hee Joo; Gee, Shirley J.; Wang, Minghua; Liu, Fengquan; Hammock, Bruce D.;

Published:

Jan 1 2014

Source:

RSC Adv. 4(80):42445-42453.

Description:

An enzyme-linked immunosorbent assay (ELISA) was developed to detect organophosphorus pesticides using a phage-borne peptide that was isolated from a cyclic 8-residue peptide phage library. The IC50 values of the phage ELISA ranged from 1.4 to 92.1 �...

File Type:

[PDF - 735.05 KB]