Diagnosis of Tuberculosis by an Enzyme-Linked Immunospot Assay for Interferon-γ

Wang, Jann-Yuan; Chou, Chien-Hong; Lee, Li-Na; Hsu, Hsiao-Leng; Jan, I-Shiow; Hsueh, Po-Ren; Yang, Pan-Chyr; Luh, Kwen-Tay

doi:10.3201/eid1304.051195

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Diagnosis of Tuberculosis by an Enzyme-Linked Immunospot Assay for Interferon-γ

Published Date:

Apr 2007

Publisher's site:

http://dx.doi.org/10.3201/eid1304.051195 New Site Icon

Source:

Emerg Infect Dis. 13(4):553-558.

[PDF-194.35 KB]

Details:

Personal Authors:

Wang, Jann-Yuan ; Chou, Chien-Hong ; Lee, Li-Na ; Hsu, Hsiao-Leng ; Jan, I-Shiow ; ... More ▼

Keywords:

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Description:

We evaluated an enzyme-linked immunospot assay for interferon-gamma (T SPOT-TB) for rapid diagnosis of active tuberculosis (TB) in a disease-endemic area. From January to June 2005, patients whose clinical symptoms and radiographic findings were compatible with TB were recruited, and a blood sample was obtained for T SPOT-TB assay within 7 days of microbiologic studies. Sixty-five patients were studied, including 39 (60%) with active TB. Thirty-five (53.8%) patients had underlying medical conditions. Thirty-seven patients had positive cultures for Mycobacterium tuberculosis, and 11 patients had positive cultures for nontuberculous mycobacteria. The sensitivity, specificity, positive predictive value, and negative predictive value of the T SPOT-TB assay were 87.2%, 88.5%, 91.9%, and 82.1%, respectively. The accuracy of this test in diagnosing active TB is >80%, even in an area with a high incidence of nontuberculous mycobacterial disease.

Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases

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