Recovery and detection of botulinum neurotoxins from a nonporous surface

Details

• Alternative Title:
  J Microbiol Methods
• Personal Author:
  Lautenschlager, Matthew ; Maslanka, Susan E. ; Paul, Paris A. ; Kalb, Suzanne R. ; Barr, John R. ; Raphael, Brian H.
• Description:
  We describe the adaptation of a sample recovery method for botulinum neurotoxins from stainless steel. Botulinum toxin was recovered from surfaces left to dry for up to 16 h and detected by either ELISA or EndoPep mass spectrometry methods. In addition, we demonstrate that this method can be used to evaluate the efficacy of surface decontamination procedures.
• Subjects:
  Botulinum Toxins Environmental Exposure Enzyme-Linked Immunosorbent Assay Mass Spectrometry Specimen Handling Stainless Steel
• Keywords:
  Decontamination ELISA Endopep-MS Environmental Microbiology Sampling
• Source:
  J Microbiol Methods. 92(3):278-280
• Pubmed ID:
  23280253
• Pubmed Central ID:
  PMC11302431
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  92
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:0c185a02fc7c204f50af4cc6980644608e207b269d8db63bcbf097fe1ddb1fad5751e16fa12a9f2ed5f22957fcc0acf100735c198afc863d28bde1f15274e30b
• Download URL:
  https://stacks.cdc.gov/view/cdc/159604/cdc_159604_DS1.pdf
• File Type:
  [PDF - 498.78 KB ]