SARS–associated Coronavirus Replication in Cell Lines

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Kaye, Matthew ; Druce, Julian ; Tran, Thomas ; Kostecki, Renata ; Chibo, Doris ; Morris, Jessica ; Catton, Mike ; Birch, Chris
• Description:
  Given the potential for laboratory-associated severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infections, we must know which cell lines are susceptible to the virus. We investigated 21 cell lines routinely used for virus isolation or research. After infection with SARS-CoV, cells were observed for cytopathic effects, and quantitative real-time polymerase chain reaction was used to measure ongoing viral replication. An indirect immunofluorescence assay was also used as a confirmatory test. The study identified 10 new cell lines capable of supporting the replication of SARS-CoV and confirmed the susceptibility of 4 cell lines previously reported. This study shows that SARS-CoV can be isolated in several cell lines commonly used for diagnostic or research purposes. It also shows that SARS-CoV can achieve high titers in several cell lines, sometimes in the absence of specific cytopathic effects.
• Subjects:
  Animals Cell Line Cell Susceptibility, Cytopathic Effects, Research Cytopathogenic Effect, Viral Dogs Haplorhini Humans Mice Rabbits Research SARS-CoV SARS Virus Sensitivity And Specificity Virus Replication

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• Source:
  Emerg Infect Dis. 12(1):128-133.
• Document Type:
  Journal Article
• Volume:
  12
• Issue:
  1
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:48ce48aa1a70c755ed947f4da5ca1b45b64dbcbe6fbc39eac47e427f1933a13b
• Download URL:
  https://stacks.cdc.gov/view/cdc/15916/cdc_15916_DS1.pdf
• File Type:
  [PDF - 360.28 KB ]