Pyrosequencing Bacillus anthracis

Wahab, Tara; Hjalmarsson, Sandra; Wollin, Ralfh; Engstrand, Lars

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Pyrosequencing Bacillus anthracis

Oct 2005
By Wahab, Tara ; Hjalmarsson, Sandra ; Wollin, Ralfh ; ...
Source: Emerg Infect Dis. 11(10):1527-1531.

[PDF-251.02 KB]

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Details:

Alternative Title:

Emerg Infect Dis
Personal Author:

Wahab, Tara ; Hjalmarsson, Sandra ; Wollin, Ralfh ; Engstrand, Lars

Wahab, Tara ; Hjalmarsson, Sandra ; Wollin, Ralfh ; Engstrand, Lars Less -
Description:

Pyrosequencing technology is a sequencing method that screens DNA nucleotide incorporation in real time. A set of coupled enzymatic reactions, together with bioluminescence, detects incorporated nucleotides in the form of light pulses, which produces a profile of characteristic peaks in a pyrogram. We used this technology to identify the warfare agent Bacillus anthracis by sequencing 4 single nucleotide polymorphisms (SNPs) in the rpoB gene as chromosomal markers for B. anthracis. In addition, 1 segment in each of the B. anthracis plasmids pXO1 and pXO2 was analyzed to determine the virulence status of the bacterial strains. Pyrosequencing technology is a powerful method to identify B. anthracis.
Subjects:

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Animals Bacillus Bacillus Anthracis Bacterial Typing Techniques DNA-Directed RNA Polymerases Genotype Humans Plasmids Polymerase Chain Reaction Polymorphism, Single Nucleotide Pyrosequencing Research RpoB Sequence Analysis, DNA Species Specificity Species Verification Virulence
Source:

Emerg Infect Dis. 11(10):1527-1531.
Document Type:

Journal Article
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

[+]

urn:sha256:963770e0f03fff79658b2399028cc900694f688d60b40dd083a8c00c2201eb98
Download URL:

https://stacks.cdc.gov/view/cdc/15800/cdc_15800_DS1.pdf
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