Pyrosequencing Bacillus anthracis

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Wahab, Tara ; Hjalmarsson, Sandra ; Wollin, Ralfh ; Engstrand, Lars
• Description:
  Pyrosequencing technology is a sequencing method that screens DNA nucleotide incorporation in real time. A set of coupled enzymatic reactions, together with bioluminescence, detects incorporated nucleotides in the form of light pulses, which produces a profile of characteristic peaks in a pyrogram. We used this technology to identify the warfare agent Bacillus anthracis by sequencing 4 single nucleotide polymorphisms (SNPs) in the rpoB gene as chromosomal markers for B. anthracis. In addition, 1 segment in each of the B. anthracis plasmids pXO1 and pXO2 was analyzed to determine the virulence status of the bacterial strains. Pyrosequencing technology is a powerful method to identify B. anthracis.
• Subjects:
  Animals Bacillus Bacillus Anthracis Bacterial Typing Techniques DNA-Directed RNA Polymerases Genotype Humans Plasmids Polymerase Chain Reaction Polymorphism, Single Nucleotide Pyrosequencing Research RpoB Sequence Analysis, DNA Species Specificity Species Verification Virulence

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• Source:
  Emerg Infect Dis. 11(10):1527-1531.
• Document Type:
  Journal Article
• Volume:
  11
• Issue:
  10
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:963770e0f03fff79658b2399028cc900694f688d60b40dd083a8c00c2201eb98
• Download URL:
  https://stacks.cdc.gov/view/cdc/15800/cdc_15800_DS1.pdf
• File Type:
  [PDF - 251.02 KB ]