Accelerated onset of diabetes in NOD mice fed a refined high-fat diet

Details

• Alternative Title:
  Diabetes Obes Metab
• Personal Author:
  Batdorf, Heidi M. ; de Luna Lawes, Luz ; Cassagne, Gabrielle A. ; Fontenot, Molly S. ; Harvey, Innocence C. ; Richardson, Jeremy T. ; Burk, David H. ; Dupuy, Samuel D. ; Karlstad, Michael D. ; Salbaum, J. Michael ; Staszkiewicz, Jaroslaw ; Beyl, Robbie ; Ghosh, Sujoy ; Burke, Susan J. ; Collier, J. Jason
• Description:
  Objective:
  
  Type 1 diabetes results from autoimmune events influenced by environmental variables, including changes in diet. This study investigated how feeding refined versus unrefined (aka ‘chow’) diets affects the onset and progression of hyperglycemia in non-obese diabetic (NOD) mice.
  
  Methods:
  
  Female NOD mice were fed either unrefined diets or matched refined low- and high-fat diets. The onset of hyperglycemia, glucose tolerance, food intake, energy expenditure, circulating insulin, liver gene expression, and microbiome changes were measured for each dietary group.
  
  Results:
  
  NOD mice consuming unrefined (chow) diets developed hyperglycemia at similar frequencies. By contrast, mice consuming the defined high-fat diet had an accelerated onset of hyperglycemia compared to the matched low-fat diet. There was no change in food intake, energy expenditure, or physical activity within each respective dietary group. Microbiome changes were driven by diet type, with chow diets clustering similarly while refined low- and high-fat bacterial diversity also grouped closely. In the defined dietary cohort, liver gene expression changes in high-fat-fed mice were consistent with a greater frequency of hyperglycemia and impaired glucose tolerance.
  
  Conclusion:
  
  Glucose intolerance is associated with enhanced frequency of hyperglycemia in female NOD mice fed a defined high-fat diet. Using an appropriate matched control diet is an essential experimental variable when studying changes in microbiome composition and diet as a modifier of disease risk.
  
  
• Subjects:
  Animals Blood Glucose Diabetes Mellitus, Type 1 Diet, Fat-Restricted Diet, High-Fat Energy Metabolism Female Glucose Intolerance Hyperglycemia Insulin Liver Mice Mice, Inbred NOD

  More +
• Keywords:
  Autoimmunity Diabetes Diet Microbiome Non-obese Diabetic (NOD) Mouse Model Obesity
• Source:
  Diabetes Obes Metab. 26(6):2158-2166
• Pubmed ID:
  38433703
• Pubmed Central ID:
  PMC11078605
• Document Type:
  Journal Article
• Funding:
  S10 OD023703/OD/NIH HHSUnited States/ ; P30 DK072476/DK/NIDDK NIH HHSUnited States/ ; R01 DK123183/DK/NIDDK NIH HHSUnited States/ ; P20 GM135002/GM/NIGMS NIH HHSUnited States/ ; S10 OD023703/CD/ODCDC CDC HHSUnited States/
• Volume:
  26
• Issue:
  6
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:746ac94796d7d307ef4c98a63cde53056284780958f3533b0f925e67f263ae8dc1f8861e38c404b9c24fef8baf09d05f2e81a3db95d3a27821e273def8aaba60
• Download URL:
  https://stacks.cdc.gov/view/cdc/155559/cdc_155559_DS1.pdf
• File Type:
  [PDF - 1.36 MB ]