Use of Diagnostic Ions for the Detection of Fentanyl Analogs in Human Matrices by LC-QTOF

Details

• Alternative Title:
  J Am Soc Mass Spectrom
• Personal Author:
  Swanson, Kenneth D. ; Shaner, Rebecca L. ; Krajewski, Logan C. ; Bragg, William A. ; Johnson, Rudolph C. ; Hamelin, Elizabeth I.
• Description:
  To combat the ongoing opioid epidemic, our laboratory has developed and evaluated an approach to detect fentanyl analogs in urine and plasma by screening LC-QTOF MS/MS spectra for ions that are diagnostic of the core fentanyl structure. MS/MS data from a training set of 142 fentanyl analogs were used to select the four product ions and six neutral losses that together provided the most complete coverage (97.2%) of the training set compounds. Furthermore, using the diagnostic ion screen against a set of 49 fentanyl analogs not in the training set resulted in 95.9% coverage of those compounds. With this approach, lower reportable limits for fentanyl and a subset of fentanyl-related compounds range from 0.25 to 2.5 ng/mL in urine and 0.5 to 5.0 ng/mL in plasma. This innovative processing method was applied to evaluate simulated exposure samples of remifentanil and carfentanil in water and their metabolites remifentanil acid and norcarfentanil in urine. This flexible approach enables a way to detect emerging fentanyl analogs in clinical samples.
• Subjects:
  Chromatography, Liquid Fentanyl Humans Ions Synthetic Drugs Tandem Mass Spectrometry
• Source:
  J Am Soc Mass Spectrom. 32(12):2852-2859
• Pubmed ID:
  34793156
• Pubmed Central ID:
  PMC10955423
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  32
• Issue:
  12
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:07daba26642ade13968cfba09b48714670c255ab96d79297c9ba0672b5ce279b
• Download URL:
  https://stacks.cdc.gov/view/cdc/153463/cdc_153463_DS1.pdf
• File Type:
  [PDF - 508.04 KB ]