rpoB Gene mutations in rifampin-resistant Mycobacterium tuberculosis identified by polymerase chain reaction single-stranded conformational polymorphism.

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Bobadilla-del-Valle, M. ; Ponce-de-Leon, A. ; Arenas-Huertero, C. ; Vargas-Alarcon, G. ; Kato-Maeda, M. ; Small, P. M. ; Couary, P. ; Ruiz-Palacios, G. M. ; Sifuentes-Osornio, J.
• Description:
  The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results. To determine the sensitivity of this method, we analyzed 35 RIFr strains and 11 rifampin-susceptible (RIFs) strains, using the DNA sequencing of the core region of rpoB for comparison. Of the RIFr, 24 had a PCR-SSCP pattern identical to that of H37Rv; the other 11 had four different patterns. The 11 RIFs had PCR-SSCP patterns identical to that of H37Rv. The sensitivity of the assay was 31.4%; its specificity was 100%. We observed a strong correlation between the degree of resistance and the type of mutation.
• Subjects:
  Antibiotics, Antitubercular DNA, Bacterial DNA-Directed Rna Polymerases Drug Resistance, Bacterial Humans Mutagenesis Mycobacterium Tuberculosis Polymerase Chain Reaction Polymorphism, Single-Stranded Conformational Rifampin Sequence Analysis, DNA

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• Source:
  Emerg Infect Dis. 7(6):1010-1013.
• Pubmed ID:
  11747730
• Pubmed Central ID:
  PMC2631921
• Document Type:
  Journal Article
• Funding:
  ERID-TW-96-001/TW/FIC NIH HHS/United States ; ICIDR-5U01AI35969/CI/NCPDCID CDC HHS/United States
• Volume:
  7
• Issue:
  6
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:1af0787027ed3b4ff6e91f4f86599c06ecda1f17d9d004eebb2e6905ccdc2990
• Download URL:
  https://stacks.cdc.gov/view/cdc/15234/cdc_15234_DS1.pdf
• File Type:
  [PDF - 42.49 KB ]