Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Mäkinen, J. ; Viljanen, M. K. ; Mertsola, J. ; Arvilommi, H. ; He, Q.
Mäkinen, J. ; Viljanen, M. K. ; Mertsola, J. ; Arvilommi, H. ; He, Q. Less -
Description:
Recently, eight allelic variants of the pertactin gene (prn1-8) have been characterized in Bordetella pertussis strains isolated in Europe and the United States. It has been suggested that the divergence of the pertactin types of clinical isolates from those of the B. pertussis vaccine strains is a result of vaccine-driven evolution. Sequencing of the prn, which is relatively time-consuming, has so far been the only method for the differentiation of prn types. We have developed a rapid real-time polymerase chain reaction assay suitable for large-scale screening of the prn type of the circulating strains. This method correctly identified the prn type of all tested 41 clinical isolates and two Finnish vaccine strains. The method is simple and reliable and provides an alternative for sequencing in pertussis research.
Subject:
[+]
Amino Acid Sequence
Bacterial Outer Membrane Proteins
Base Sequence
Bordetella Pertussis
DNA, Bacterial
DNA Probes
Electrophoresis, Agar Gel
Genes, Bacterial
Genetic Variation
Humans
Molecular Sequence Data
Pertussis Vaccine
Polymerase Chain Reaction
Time Factors
Virulence Factors, Bordetella

Source:
Emerg Infect Dis. 7(6):952-958.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:10847388e73754b3650228b671b0f5085db0e2ba068399fe88cf4115efbc5bcc
File Type:

Supporting Files

• 	11747721.nxml	txt
  	license.txt	txt

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