DETECTION OF SARS-COV-2 NEUTRALIZING ANTIBODIES IN RETROPHARYNGEAL LYMPH NODE EXUDATES OF WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS) FROM NEBRASKA, USA

Details

• Alternative Title:
  J Wildl Dis
• Personal Author:
  Poonsuk, Korakrit ; Loy, Duan ; Birn, Rachael ; Buss, Bryan ; Donahue, Matthew ; Nordeen, Todd ; Sinclair, Kylie ; Meduna, Luke ; Brodersen, Bruce ; Loy, John Dustin
• Description:
  Disease surveillance testing for emerging zoonotic pathogens in wildlife is a key component in understanding the epidemiology of these agents and potential risk to human populations. Recent emergence of SARS-CoV-2 in humans, and subsequent detection of this virus in wildlife, highlights the need for developing new One Health surveillance strategies. We used lymph node exudate, a sample type that is routinely collected in hunter-harvested white-tailed deer (WTD, Odocoileus virginianus) for surveillance of chronic wasting disease, to assess anti-SARS-CoV-2 neutralizing antibodies. A total of 132 pairs of retropharyngeal lymph nodes collected from Nebraska WTD harvested in Nebraska, US, in 2019 (pre-SARS-CoV-2 pandemic) and 2021 (post-SARS-CoV-2 pandemic) were tested for SARS-CoV-2 with reverse transcription PCR. Thereafter, exudates obtained from these same lymph nodes were tested for SARS-CoV-2 neutralizing antibodies using a surrogate virus neutralization test. Neutralizing antibodies were detected in the exudates with high diagnostic specificity (100% at proposed cutoff of 40% inhibition). Application of this testing approach to samples collected for use in other disease surveillance activities may provide additional epidemiological data on SARS-CoV-2 exposure, and there is further potential to apply this sample type to detection of other pathogens of interest.
• Subjects:
  Animals Animals, Wild Antibodies, Neutralizing Antibodies, Viral COVID-19 Deer Electron Spin Resonance Spectroscopy Humans Lymph Nodes Nebraska SARS-CoV-2

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• Keywords:
  Lymph Node Neutralizing Antibody Serology
• Source:
  J Wildl Dis. 59(4):702-708
• Pubmed ID:
  37768779
• Pubmed Central ID:
  PMC10913095
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  59
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:ec7674667f780d7cb4da4896264cc690ee28fc37d3fdca5e752d71ca8662dc860470f8509a5fc47f9f402728840338785dbe388a7a79a1c599ffb0e56d60a638
• Download URL:
  https://stacks.cdc.gov/view/cdc/150411/cdc_150411_DS1.pdf
• File Type:
  [PDF - 443.47 KB ]