Toxin and phage production from pathogenic E. coli by antibiotic induction analyzed by chemical reduction, MALDI-TOF-TOF mass spectrometry and top-down proteomic analysis

Details

• Personal Author:
  Fagerquist, Clifton K. ; Shi, Yanlin ; Dodd, Claire E.
• Description:
  Shiga toxin-producing Escherichia coli (STEC) are an ongoing threat to public health and agriculture. Our laboratory has developed a rapid method for identification of Shiga toxin (Stx), bacteriophage, and host proteins produced from STEC. We demonstrate this technique on two genomically sequenced STEC O145:H28 strains linked to two major outbreaks of foodborne illness occurring in 2007 (Belgium) and 2010 (Arizona).
• Subjects:
  Bacteriophages Escherichia Coli Proteins
• Pubmed ID:
  36905351
• Document Type:
  Journal Article
• Funding:
  2030-42000-055-000-D/Agricultural Research Service: USDA-ARS Project Number
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:e6e9e4dc0ad2d813713b847b1f8d61330507b2596b2c1c137594dbc010aa4633
• Download URL:
  https://stacks.cdc.gov/view/cdc/149135/cdc_149135_DS1.pdf
• File Type:
  [PDF - 1.73 MB ]