Clonal differences among erythromycin-resistant Streptococcus pyogenes in Spain.

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Perez-Trallero, E. ; Marimón, J. M. ; Montes, M. ; Orden, B. ; de Pablos, M.
• Description:
  The aim of this study was to determine whether the high levels of erythromycin resistance in Streptococcus pyogenes found in Spain are due to the introduction and spread of one or more clones. Phenotypic and genotypic techniques were used to characterize all erythromycin-resistant S. pyogenes (ErR) isolated in Gipuzkoa, Spain, in the last 10 years and 128 ErR isolated in Vitoria and Madrid during 1996. Of 437 ErR, 97% had the M phenotype; all 283 of the strains studied had the mefA determinant of resistance. After biotyping, T serotyping, emm typing, and genotyping, four major clones were detected. Clones B (biotype I, type T4, emm4, pulsed-field gel electrophoresis [PFGE] II) and D (biotype V, type T8.25, emm75, PFGE IV) comprised 78.8% of all ErR. The resistance of S. pyogenes to erythromycin was mainly due to an efflux mechanism of resistance (M phenotype); few clones were responsible for it.
• Subjects:
  Anti-Bacterial Agents Bacterial Typing Techniques Drug Resistance, Microbial Electrophoresis, Gel, Pulsed-Field Erythromycin Streptococcus Pyogenes
• Source:
  Emerg Infect Dis. 5(2):235-240.
• Document Type:
  Journal Article
• Place as Subject:
  Spain
• Volume:
  5
• Issue:
  2
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:708fa1c4482821600ab96bb279b205b2c181ef1e2a586b76e391058048fe28d1
• Download URL:
  https://stacks.cdc.gov/view/cdc/14811/cdc_14811_DS1.pdf
• File Type:
  [PDF - 107.39 KB ]