Genetic and Transmission Analysis of Helicobacter pylori Strains within a Family1

Raymond, Josette; Thiberge, Jean-Michel; Chevalier, Catherine; Kalach, Nicolas; Bergeret, Michel; Labigne, Agnès; Dauga, Catherine

i

Genetic and Transmission Analysis of Helicobacter pylori Strains within a Family1

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Oct 2004
By Raymond, Josette ; Thiberge, Jean-Michel ; Chevalier, Catherine ; ...

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Raymond, Josette ; Thiberge, Jean-Michel ; Chevalier, Catherine ; Kalach, Nicolas ; Bergeret, Michel ; Labigne, Agnès ; Dauga, Catherine
Description:

To look for evidence of intrafamilial infection, we isolated 107 Helicobacter pylori clones from biopsied specimens taken from both parents and four children. We compared the sequences of two housekeeping genes (hspA and glmM) from these clones with those of 131 unrelated strains from patients living in different geographic regions. Strain relationships within the family were determined by analyzing allelic variation at both loci and building phylogenetic trees and by using multilocus sequence typing. Both hspA- and glmM-based phylogenetic trees showed East Asian and African branches. All samples from family members showed natural mixed infection. Identical alleles found in some strains isolated from the children and parents, but not in the strains isolated from unrelated patients, demonstrated that strains have circulated within the family. Several mechanisms, such as point mutations, intragenic recombination, and introduction of foreign (African) alleles, were shown to enhance strain diversity within the family.
Subjects:

Adolescent Adult Amino Acid Sequence Bacterial Typing Techniques Base Sequence Child Child, Preschool Epidemiology Family Health Family Transmission Female Gastritis Genes, Bacterial Genetic Diversity Genetic Variation Helicobacter Infections Helicobacter Pylori Humans Male Molecular Sequence Data Phylogeny Research Sequence Homology, Amino Acid
Source:

Emerg Infect Dis. 10(10):1816-1821.
Document Type:

Journal Article
Volume:

10
Issue:

10
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:49c9e60f89696e5a3ca12f4054e50376dac34227ccad9b8155d609a2f2dce0d6
Download URL:

https://stacks.cdc.gov/view/cdc/14589/cdc_14589_DS1.pdf
File Type:

[PDF - 266.29 KB ]

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