Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan

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• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Wu, Ho-Sheng ; Chiu, Shu-Chun ; Tseng, Tsan-Chang ; Lin, Szu-Fong ; Lin, Jih-Hui ; Hsu, Yu-Fen ; Wang, Mei-Ching ; Lin, Tsuey-Li ; Yang, Wen-Zieh ; Ferng, Tian-Lin ; Huang, Kai-Hung ; Hsu, Li-Ching ; Lee, Li-Li ; Yang, Jyh-Yuan ; Chen, Hour-Young ; Su, Shun-Pi ; Yang, Shih-Yan ; Lin, Ting-Hsiang ; Su, Ih-Jen
• Description:
  Severe acute respiratory syndrome (SARS) has raised a global alert since March 2003. After its causative agent, SARS-associated coronavirus (SARS-CoV), was confirmed, laboratory methods, including virus isolation, reverse transcriptase-polymerase chain reaction (RT-PCR), and serologic methods, have been quickly developed. In this study, we evaluated four serologic tests ( neutralization test, enzyme-linked immunosorbent assay [ELISA], immunofluorescent assay [IFA], and immunochromatographic test [ICT]) for detecting antibodies to SARS-CoV in sera of 537 probable SARS case-patients with correlation to the RT-PCR. With the neutralization test as a reference method, the sensitivity, specificity, positive predictive value, and negative predictive value were 98.2%, 98.7%, 98.7%, and 98.4% for ELISA; 99.1%, 87.8%, 88.1% and 99.1% for IFA; 33.6%, 98.2%, 95.7%, and 56.1% for ICT, respectively. We also compared the recombinant-based western blot with the whole virus-based IFA and ELISA; the data showed a high correlation between these methods, with an overall agreement of >90%. Our results provide a systematic analysis of serologic and molecular methods for evaluating SARS-CoV infection.
• Subjects:
  Antibodies, Viral Antigens, Viral Blotting, Western Chromatography Cross Reactions Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Humans Neutralization Tests Predictive Value Of Tests Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction SARS Virus Sensitivity And Specificity Severe Acute Respiratory Syndrome Taiwan

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• Source:
  Emerg Infect Dis. 10(2):305-310.
• Document Type:
  Journal Article
• Volume:
  10
• Issue:
  2
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:5d74d0cb09090e1ab6885b0c18c0c14ffe40db81b9713e3a2e6fa0a83e975baa
• Download URL:
  https://stacks.cdc.gov/view/cdc/14494/cdc_14494_DS1.pdf
• File Type:
  [PDF - 383.62 KB ]