Immunofluorescence Assay for Serologic Diagnosis of SARS
Published Date:Mar 2004
Source:Emerg Infect Dis. 10(3):530-532.
Communicable Diseases, Emerging
Fluorescent Antibody Technique, Indirect
Reverse Transcriptase Polymerase Chain Reaction
Severe Acute Respiratory Syndrome
Description:We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
You May Also Like: