Immunofluorescence Assay for Serologic Diagnosis of SARS

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Chan, Paul K. S. ; Ng, King-Cheung ; Chan, Rickjason C. W. ; Lam, Rebecca K. Y. ; Chow, Viola C. Y. ; Hui, Mamie ; Wu, Alan ; Lee, Nelson ; Yap, Florence H.Y. ; Cheng, Frankie W. T. ; Sung, Joseph J. Y. ; Tam, John S.
• Description:
  We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
• Subjects:
  Adolescent Adult Antibodies, Viral Child Child, Preschool Communicable Diseases, Emerging Coronavirus Diagnosis Dispatch Female Fluorescent Antibody Technique, Indirect Hong Kong Humans Male Middle Aged Reverse Transcriptase Polymerase Chain Reaction SARS Sensitivity Serology Severe Acute Respiratory Syndrome Specificity

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• Source:
  Emerg Infect Dis. 10(3):530-532.
• Document Type:
  Journal Article
• Volume:
  10
• Issue:
  3
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:4b618b921088bb9e2702bfbefff651b2a91429059a9b65a38b9c8747308be9ed
• Download URL:
  https://stacks.cdc.gov/view/cdc/14408/cdc_14408_DS1.pdf
• File Type:
  [PDF - 96.04 KB ]