Immunofluorescence Assay for Serologic Diagnosis of SARS

Chan, Paul K. S.; Ng, King-Cheung; Chan, Rickjason C. W.; Lam, Rebecca K. Y.; Chow, Viola C. Y.; Hui, Mamie; Wu, Alan; Lee, Nelson; Yap, Florence H.Y.; Cheng, Frankie W. T.; Sung, Joseph J. Y.; Tam, John S.

doi:10.3201/eid1003.030493

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Immunofluorescence Assay for Serologic Diagnosis of SARS

Mar 2004
By Chan, Paul K. S. ; Ng, King-Cheung ; Chan, Rickjason C. W. ; ...
http://dx.doi.org/10.3201/eid1003.030493
Source: Emerg Infect Dis. 10(3):530-532.

[PDF-96.04 KB]

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Details:

Alternative Title:

Emerg Infect Dis
Personal Author:

Chan, Paul K. S. ; Ng, King-Cheung ; Chan, Rickjason C. W. ; Lam, Rebecca K. Y. ; Chow, Viola C. Y. ; ... More +

Chan, Paul K. S. ; Ng, King-Cheung ; Chan, Rickjason C. W. ; Lam, Rebecca K. Y. ; Chow, Viola C. Y. ; Hui, Mamie ; Wu, Alan ; Lee, Nelson ; Yap, Florence H.Y. ; Cheng, Frankie W. T. ; Sung, Joseph J. Y. ; Tam, John S. Less -
Description:

We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
Subjects:

[+]

Adolescent Adult Antibodies, Viral Child Child, Preschool Communicable Diseases, Emerging Coronavirus Diagnosis Dispatch Female Fluorescent Antibody Technique, Indirect Hong Kong Humans Male Middle Aged Reverse Transcriptase Polymerase Chain Reaction SARS Sensitivity Serology Severe Acute Respiratory Syndrome Specificity
Source:

Emerg Infect Dis. 10(3):530-532.
Document Type:

Journal Article
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

[+]

urn:sha256:4b618b921088bb9e2702bfbefff651b2a91429059a9b65a38b9c8747308be9ed
Download URL:

https://stacks.cdc.gov/view/cdc/14408/cdc_14408_DS1.pdf
File Type:

	03-0493.nxml	txt
	license.txt	txt

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