Molecular Analysis of Echovirus 13 Isolates and Aseptic Meningitis, Spain
Published Date:Aug 2003
Source:Emerg Infect Dis. 9(8):934-941.
Keywords:Amino Acid Sequence
Echovirus, Echovirus 13, Meningitis, Viral
Enterovirus B, Human
Meningitis, Aseptic, Molecular Epidemiology, Spain, Canary Islands , Research
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Funding:AI51202/AI/NIAID NIH HHS/United States
NS29425/NS/NINDS NIH HHS/United States
Description:Echovirus 13 (EV13), considered rare, was reported worldwide in 2000, mostly related to aseptic meningitis outbreaks. In Spain, 135 EV13 isolates were identified. The genetic relationships between 64 representative strains from Spain and other reported isolates from the United States, Germany, Italy, Japan, and Sweden were described by analyzing the partial sequence of the major capsid protein (VP1) gene. The strains from Spain were clearly identified as EV13 (79.5% similarity with the EV13 reference strain) and were grouped phylogenetically into two different clusters (by origination on either the Iberian Peninsula or Canary Islands). Isolates from Germany from 2000 clustered with the Canary Islands group. The isolates from other countries obtained before 2000 were genetically distant. Changes in EV13 coding sequence involved several differences in the C-terminal extreme of the VP1 protein. Part of the neutralizing antigenic site III has been described in this genome region in poliovirus and swine vesicular disease virus.
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