First Isolation of West Nile virus from a Patient with Encephalitis in the United States

Huang, Cinnia; Slater, Brett; Rudd, Robert; Parchuri, Nandakishore; Hull, Rene; Dupuis, Michelle; Hindenburg, Alexander

doi:10.3201/eid0812.020532

i

First Isolation of West Nile virus from a Patient with Encephalitis in the United States

Supporting Files

Dec 2002
By Huang, Cinnia ; Slater, Brett ; Rudd, Robert ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Huang, Cinnia ; Slater, Brett ; Rudd, Robert ; Parchuri, Nandakishore ; Hull, Rene ; Dupuis, Michelle ; Hindenburg, Alexander
Description:

West Nile virus (WNV) was isolated from a patient who developed encephalitis while undergoing treatment with CHOP (cyclophosphamide, hydroxydoxorubicin, vincristine [Oncovin], predisone) and rituximab for a non-Hodgkin B-cell lymphoma. Both standard reverse transcription-polymerase chain reaction (RT-PCR) and Taqman RT-PCR established the diagnosis of WNV infection from cerebrospinal fluid (CSF). Several whole blood samples and one serum sample underwent further testing. CSF and serum samples were negative for WNV antibody; however, all samples were positive by both RT-PCR assays. Infectious virus was recovered from a blood sample, and its identity was confirmed by using a WNV-specific immunofluorescence assay. The complete WNV genomes determined from CSF and from the virus isolate adapted from cell culture were the same. The results represent the first complete WNV genome sequence obtained directly from human CSF and the first time that infectious WNV has been recovered from a patient with encephalitis in North America.
Subjects:

Aged Antineoplastic Combined Chemotherapy Protocols Cyclophosphamide Doxorubicin Fatal Outcome Female Humans Lymphoma, B-Cell Prednisone Research Vincristine West Nile Fever West Nile Virus
Source:

Emerg Infect Dis. 8(12):1367-1371.
Document Type:

Journal Article
Funding:

U50/CCU212415-04/CC/ODCDC CDC HHS/United States ; U50/CCU213698-03/CC/ODCDC CDC HHS/United States
Place as Subject:

New York
Location:

North America
Volume:

8
Issue:

12
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:d8366f240f00e1d08ffd6dc1d231d8be826d8d283657685878c93ea415a2af46
Download URL:

https://stacks.cdc.gov/view/cdc/14110/cdc_14110_DS1.pdf
File Type:

[PDF - 490.31 KB ]

File Language:

English

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