Molecular Classification of Enteroviruses Not Identified by Neutralization Tests

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Kubo, Hideyuki ; Iritani, Nobuhiro ; Seto, Yoshiyuki
• Description:
  We isolated six viruses from patients diagnosed with aseptic meningitis or hand, foot, and mouth disease. The cytopathic effect of these viruses on cultured cells was like that of enteroviruses. However, viral neutralization tests against standard antisera were negative. Phylogenetic analysis with the complete VP4 nucleotide sequences of these 6 viruses and 29 serotypes of enteroviruses classified 3 of the viruses as serotype echovirus type 18 (EV18) and 3 as serotype human enterovirus 71 (HEV71). These results were confirmed by remicroneutralization tests with HEV-monospecific antisera or an additional phylogenetic analysis with the complete VP4 nucleotide sequences. Phylogenetic analysis with complete VP4 genes is more useful than neutralization tests with enterovirus serotype-specific antisera in identifying enterovirus serotypes.
• Subjects:
  Enterovirus Humans Meningitis, Viral Molecular Biology Neutralization Tests Phylogeny Polymerase Chain Reaction Serotyping
• Source:
  Emerg Infect Dis. 8(3):298-304.
• Document Type:
  Journal Article
• Place as Subject:
  Japan
• Location:
  Asia
• Volume:
  8
• Issue:
  3
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:72cb36c8e8873dd0591bdb4d8a0a49a9609e8dffb09bb7326c9f8c9585998fbc
• Download URL:
  https://stacks.cdc.gov/view/cdc/13783/cdc_13783_DS1.pdf
• File Type:
  [PDF - 176.33 KB ]