Traditional and Molecular Techniques for the Study of Emerging Bacterial Diseases: One Laboratory’s Perspective

Houpikian, Pierre; Raoult, Didier

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Traditional and Molecular Techniques for the Study of Emerging Bacterial Diseases: One Laboratory’s Perspective

Supporting Files Public Domain

Feb 2002
By Houpikian, Pierre ; Raoult, Didier

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Houpikian, Pierre ; Raoult, Didier
Description:

Identification of emerging bacterial pathogens generally results from a chain of events involving microscopy, serology, molecular tools, and culture. Because of the spectacular molecular techniques developed in the last decades, some authors think that these techniques will shortly supplant culture. The key steps that led to the discovery of emerging bacteria have been reviewed to determine the real contribution of each technique. Historically, microscopy has played a major role. Serology provided indirect evidence for causality. Isolation and culture were crucial, as all emerging bacteria have been grown on artificial media or cell lines or at least propagated in animals. With the use of broad-range polymerase chain reaction, some bacteria have been identified or detected in new clinical syndromes. Culture has irreplaceable advantages for studying emerging bacterial diseases, as it allows antigenic studies, antibiotic susceptibility testing, experimental models, and genetic studies to be carried out, and remains the ultimate goal of pathogen identification.
Subjects:

16S RDNA Animal Model Animals Antibodies, Bacterial Bacteria Bacterial Detection Bacterial Identification Bacterial Infections Broad-range PCR Complete Nucleotide Sequence Culture Culture Media DNA, Bacterial Emerging Bacteria Histologic Stain Humans Laboratories Microscopy Models, Animal Molecular Diagnostic Techniques Perspective Sensitivity And Specificity Sequence Analysis, DNA Unrecognized Pathogens
Source:

Emerg Infect Dis. 8(2):122-131.
Document Type:

Journal Article
Volume:

8
Issue:

2
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:3e80b824e9f8b5328a788a4f264afd91d195e127df04fdad1a59062afda2dc48
Download URL:

https://stacks.cdc.gov/view/cdc/13760/cdc_13760_DS1.pdf
File Type:

[PDF - 134.58 KB ]

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