Details:

Alternative Title:
Andrology
Personal Author:
Voigt, Anna Laura ; de Lima e Martins Lara, Nathalia ; Dobrinski, Ina
Voigt, Anna Laura ; de Lima e Martins Lara, Nathalia ; Dobrinski, Ina Less -
Description:
Background:

Survivors of childhood cancer often suffer from infertility. While sperm cryopreservation is not feasible before puberty, the patient’s own spermatogonial stem cells (SSCs), could serve as a germ cell reservoir, enabling these patients to father their own children in adulthood through the isolation, in vitro expansion, and subsequent transplantation of SSCs. However, this approach requires large numbers of stem cells and methods for successfully propagating SSCs in the laboratory are yet to be established for higher mammals and humans. The improvement of SSC culture requires deeper understanding of their metabolic requirements and the mechanisms that regulate metabolic homeostasis.

Aim:

This review gives a summary on our knowledge of SSC metabolism during maintenance and differentiation and highlights the potential influence of Sertoli cell and stem cell niche maturation on SSC metabolic requirements during development.

Results and Conclusions:

Fetal human SSC precursors, or gonocytes, migrate into the seminiferous cords and supposedly mature to adult stem cells within the first year of human development. However, the SSC niche doesn’t fully differentiate until puberty, when Sertoli cells dramatically rearrange the architecture and microenvironment within the seminiferous epithelium. Consequently, prepubertal and adult SSCs experience two distinct niche environments potentially affecting SSC metabolism and maturation. Indeed, the metabolic requirements of mouse PGCs and pig gonocytes are distinct from their adult counterparts and novel single cell RNA sequencing analysis of human and porcine SSCs during development confirms this metabolic transition. Knowledge of the metabolic requirements and their changes and regulation during SSC maturation is necessary to implement laboratory-based techniques and enable clinical use of SSCs. Based on the advancement in our understanding of germline metabolism circuits and maturation events of niche cells within the testis we propose a new definition of spermatogonial stem cell maturation and its amendment in the light of metabolic change.


Subjects:
[+]
Article Development Metabolism Microenvironment Niche Maturation Testis
Source:
Andrology.
Pubmed ID:
36690000
Pubmed Central ID:
PMC10363251
Document Type:
Journal Article
Funding:
R01 HD091068/HD/NICHD NIH HHSUnited States/ ; R01 OD016575/OD/NIH HHSUnited States/ ; R01 OD016575/CD/ODCDC CDC HHSUnited States/
R01 HD091068/HD/NICHD NIH HHSUnited States/ ; R01 OD016575/OD/NIH HHSUnited States/ ; R01 OD016575/CD/ODCDC CDC HHSUnited States/ Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:0ce128b4fc0904a4c66c54c57ec41c201d08443fbd1ce8f652526b4e45f7b3e2
File Type:

• Supporting Files:
  No Additional Files

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