Synthesis of a Bifunctionalized Glycosylphosphatidylinositol (GPI) Anchor Useful for the Study of GPI Biology

Details

• Alternative Title:
  Chemistry
• Personal Author:
  Mullapudi, Venkanna Babu ; Craig, Kendall C. ; Guo, Zhongwu
• Description:
  A new, bifunctional glycosylphosphatidylinositol (GPI) derivative containing the highly conserved core structure of all natural GPI anchors with a photoactivable diazirine in the lipid chain and clickable alkynes in the glycan was synthesized by a convergent 3+2 glycosylation strategy with late stage protecting group manipulation and regioselective phosphorylation. The challenges of this synthesis were due to the presence of several distinctive functional groups in the synthetic target, which complicated the protection tactics, in addition to the inherent difficulties associated with GPI synthesis. This bifunctional GPI derivative can cross-react with molecules in proximity upon photoactivation and be subsequently labeled with other molecular tags via click reaction. Therefore, it should be a valuable probe for biological studies of GPIs, such as analysis of GPI-interacting membrane proteins, and gaining insights into their functional mechanisms.
• Subjects:
  • Biology
  • Glycosylation
  • Glycosylphosphatidylinositols
  • Membrane Proteins
  • Phosphorylation
• Keywords:
  • glycosylphosphatidylinositol
  • carbohydrate
  • glycolipid
  • photoactivation
  • click reaction
• Source:
  Chemistry. 29(17):e202203457
• Pubmed ID:
  36445784
• Pubmed Central ID:
  PMC10038835
• Document Type:
  Text
• Funding:
  R35 GM131686/GM/NIGMS NIH HHSUnited States/ ; S10 OD021758/OD/NIH HHSUnited States/ ; S10 OD021758/CD/ODCDC CDC HHSUnited States/
• Genre:
  Journal Article
• Volume:
  29
• Issue:
  17
• Download URL:
  https://stacks.cdc.gov/view/cdc/126198/cdc_126198_DS1.pdf
• File Type:
  [PDF - 808.01 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:22b1e30b285bedbfd812166302bca2c1f59f044c333160ade47b362d73424541135564f1c76332a5f440d1f7f7243b010c793647a07fab138bd9c857fbb01020