A systematic assessment of cell type deconvolution algorithms for DNA methylation data

Details

• Alternative Title:
  Brief Bioinform
• Personal Author:
  Song, Junyan ; Kuan, Pei-Fen
• Description:
  We performed systematic assessment of computational deconvolution methods that play an important role in the estimation of cell type proportions from bulk methylation data. The proposed framework methylDeConv (available as an R package) integrates several deconvolution methods for methylation profiles (Illumina HumanMethylation450 and MethylationEPIC arrays) and offers different cell-type-specific CpG selection to construct the extended reference library which incorporates the main immune cell subsets, epithelial cells and cell-free DNAs. We compared the performance of different deconvolution algorithms via simulations and benchmark datasets and further investigated the associations of the estimated cell type proportions to cancer therapy in breast cancer and subtypes in melanoma methylation case studies. Our results indicated that the deconvolution based on the extended reference library is critical to obtain accurate estimates of cell proportions in non-blood tissues.
• Keywords:
  Algorithms Cell Type Heterogeneity Deconvolution DNA Methylation Epigenetics EWAS Gene Library Humans Neoplasms
• Source:
  Brief Bioinform. 23(6)
• Pubmed ID:
  36242584
• Pubmed Central ID:
  PMC9947552
• Document Type:
  Journal Article
• Funding:
  U01 OH011478/OH/NIOSH CDC HHSUnited States/ ; U01 OH012257/OH/NIOSH CDC HHSUnited States/ ; U01OH011478/ACL HHSUnited States/ ; U01 OH011478/OH/NIOSH CDC HHSUnited States/
• Volume:
  23
• Issue:
  6
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:66f5446ded02857be1a9fdf9f6324ecfbf30dd7af9116012fdb80b8f748a042e
• Download URL:
  https://stacks.cdc.gov/view/cdc/125104/cdc_125104_DS1.pdf
• File Type:
  [PDF - 2.07 MB ]