Development of Differentiating Infected from Vaccinated Animals (DIVA) Real-Time PCR for African Horse Sickness Virus Serotype 1

Wang, Yifan; Ong, Jasmine; Ng, Oi Wing; Songkasupa, Tapanut; Koh, Eileen Y.; Wong, Jeslyn P.S.; Puangjinda, Kanokwan; Fernandez, Charlene Judith; Huangfu, Taoqi; Ng, Lee Ching; Chang, Siow Foong; Yap, Him Hoo

doi:10.3201/eid2812.220594

i

Development of Differentiating Infected from Vaccinated Animals (DIVA) Real-Time PCR for African Horse Sickness Virus Serotype 1

Supporting Files Public Domain

12 2022
By Wang, Yifan ; Ong, Jasmine ; Ng, Oi Wing ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Wang, Yifan ; Ong, Jasmine ; Ng, Oi Wing ; Songkasupa, Tapanut ; Koh, Eileen Y. ; Wong, Jeslyn P.S. ; Puangjinda, Kanokwan ; Fernandez, Charlene Judith ; Huangfu, Taoqi ; Ng, Lee Ching ; Chang, Siow Foong ; Yap, Him Hoo
Description:

African horse sickness (AHS) is a highly infectious and often fatal disease caused by 9 serotypes of the orbivirus African horse sickness virus (AHSV). In March 2020, an AHS outbreak was reported in Thailand in which AHSV serotype 1 was identified as the causative agent. Trivalent live attenuated vaccines serotype 1, 3, and 4 were used in a targeted vaccination campaign within a 50-km radius surrounding the infected cases, which promptly controlled the spread of the disease. However, AHS-like symptoms in vaccinated horses required laboratory diagnostic methods to differentiate infected horses from vaccinated horses, especially for postvaccination surveillance. We describe a real-time reverse transcription PCR-based assay for rapid characterization of the affecting field strain. The development and validation of this assay should imbue confidence in differentiating AHS-vaccinated horses from nonvaccinated horses. This method should be applied to determining the epidemiology of AHSV in future outbreaks.
Subjects:

African Horse Sickness/prevention & Control African Horse Sickness Virus Animals Horses Orbivirus Real-Time Polymerase Chain Reaction Serogroup Vaccines, Attenuated
Source:

Emerg Infect Dis. 2022; 28(12):2446-2454
Pubmed ID:

36417933
Pubmed Central ID:

PMC9707579
Document Type:

Journal Article
Place as Subject:

Thailand
Volume:

28
Issue:

12
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:69cbf8bf178ac4b23fba85965db97fc2bf8b5cf4d4dd87a6e81f86c11bd9fce1
Download URL:

https://stacks.cdc.gov/view/cdc/123306/cdc_123306_DS1.pdf
File Type:

[PDF - 2.86 MB ]

File Language:

English

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