Details:

Alternative Title:
Emerg Infect Dis
Publisher's site:
http://dx.doi.org/10.3201/eid2812.220594
Personal Author:
Wang, Yifan ; Ong, Jasmine ; Ng, Oi Wing ; Songkasupa, Tapanut ; Koh, Eileen Y. ; ... More +
Wang, Yifan ; Ong, Jasmine ; Ng, Oi Wing ; Songkasupa, Tapanut ; Koh, Eileen Y. ; Wong, Jeslyn P.S. ; Puangjinda, Kanokwan ; Fernandez, Charlene Judith ; Huangfu, Taoqi ; Ng, Lee Ching ; Chang, Siow Foong ; Yap, Him Hoo Less -
Description:
African horse sickness (AHS) is a highly infectious and often fatal disease caused by 9 serotypes of the orbivirus African horse sickness virus (AHSV). In March 2020, an AHS outbreak was reported in Thailand in which AHSV serotype 1 was identified as the causative agent. Trivalent live attenuated vaccines serotype 1, 3, and 4 were used in a targeted vaccination campaign within a 50-km radius surrounding the infected cases, which promptly controlled the spread of the disease. However, AHS-like symptoms in vaccinated horses required laboratory diagnostic methods to differentiate infected horses from vaccinated horses, especially for postvaccination surveillance. We describe a real-time reverse transcription PCR-based assay for rapid characterization of the affecting field strain. The development and validation of this assay should imbue confidence in differentiating AHS-vaccinated horses from nonvaccinated horses. This method should be applied to determining the epidemiology of AHSV in future outbreaks.
Subject:
[+]
African Horse Sickness/prevention & Control
African Horse Sickness Virus
Animals
Horses
Orbivirus
Real-Time Polymerase Chain Reaction
Serogroup
Vaccines, Attenuated

Source:
Emerg Infect Dis. 2022; 28(12):2446-2454
Pubmed ID:
36417933
Pubmed Central ID:
PMC9707579
Document Type:
Journal Article
Place as Subject:
Thailand
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:69cbf8bf178ac4b23fba85965db97fc2bf8b5cf4d4dd87a6e81f86c11bd9fce1
File Type:

Supporting Files

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  	22-0594-Techapp-s1.pdf	pdf
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