Emergence and Evolutionary Response of Vibrio cholerae to Novel Bacteriophage, Democratic Republic of the Congo1

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Alam, Meer T. ; Mavian, Carla ; Paisie, Taylor K. ; Tagliamonte, Massimiliano S. ; Cash, Melanie N. ; Angermeyer, Angus ; Seed, Kimberley D. ; Camilli, Andrew ; Maisha, Felicien Masanga ; Senga, R. Kabangwa Kakongo ; Salemi, Marco ; Morris, J. Glenn ; Ali, Afsar
• Description:
  Cholera causes substantial illness and death in Africa. We analyzed 24 toxigenic Vibrio cholerae O1 strains isolated in 2015-2017 from patients in the Great Lakes region of the Democratic Republic of the Congo. Strains originating in southern Asia appeared to be part of the T10 introduction event in eastern Africa. We identified 2 main strain lineages, most recently a lineage corresponding to sequence type 515, a V. cholerae cluster previously reported in the Lake Kivu region. In 41% of fecal samples from cholera patients, we also identified a novel ICP1 (Bangladesh cholera phage 1) bacteriophage, genetically distinct from ICP1 isolates previously detected in Asia. Bacteriophage resistance occurred in distinct clades along both internal and external branches of the cholera phylogeny. This bacteriophage appears to have served as a major driver for cholera evolution and spread, and its appearance highlights the complex evolutionary dynamic that occurs between predatory phage and bacterial host.
• Subjects:
  Bacteriophages Cholera Humans Phylogeny Vibrio Cholerae O1
• Source:
  Emerg Infect Dis. 2022; 28(12):2482-2490
• Pubmed ID:
  36417939
• Pubmed Central ID:
  PMC9707599
• Document Type:
  Journal Article
• Funding:
  R01 AI138554/AI/NIAID NIH HHSUnited States/
• Place as Subject:
  Democratic Republic of the Congo ; United States
• Volume:
  28
• Issue:
  12
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:7079e0a7d08ec779cd2a535a5c860c62de15e39b5b5ecb3747e70597afa22278
• Download URL:
  https://stacks.cdc.gov/view/cdc/123305/cdc_123305_DS1.pdf
• File Type:
  [PDF - 3.17 MB ]