Associations between Escherichia coli O157 shedding and the faecal microbiota of dairy cows

Details

• Alternative Title:
  J Appl Microbiol
• Personal Author:
  Stenkamp-Strahm, C. ; McConnel, C. ; Magzamen, S. ; Abdo, Z. ; Reynolds, S.
• Description:
  Aims ; Dairy cattle shed pathogenic Escherichia coli O157 (O157) in faeces, playing a role in human exposure. We aimed to measure faecal microbial communities in early lactation dairy cattle, and model outcomes with O157 shedding metrics. ; Methods and Results ; Daily faecal samples were collected from 40 cattle on two Colorado dairies for five consecutive days, and characterized for O157. 16S rRNA gene sequencing was used to measure sample-level microbial communities. Alpha-diversity metrics were associated with O157 outcomes via regression modelling, adjusting for confounders. Differential abundance of taxa were identified between O157(+) and O157(−) samples and between shedding days of individuals, using matched Wilcoxon rank-sum tests, zero-inflated Gaussian (ZIG) regression and negative binomial regression. After removing an outlier, multi-day and intermittently shedding cows had lower average richness compared to those that never shed. ZIG modelling revealed Bacillus coagulans to be more abundant in O157(−) samples, while Moryella were more abundant in O157(+) samples. Negative binomial models and Wilcoxon tests revealed no differentially abundant taxa between O157(+) vs O157(−) samples, or between shedding days of individuals. ; Conclusions ; Microbial diversity and some taxa may be influenced by or affect O157 shedding by dairy cattle. ; Significance and Impact of the Study ; If future work corroborates these findings, dairy cow microbial community changes may be used to guide on-farm strategies that mitigate O157 dissemination, protecting the human food chain.
• Subjects:
  • Animals
  • Cattle
  • Cattle Diseases
  • Escherichia coli Infections
  • Escherichia coli O157
  • Feces
  • Female
  • Gastrointestinal Microbiome
• Source:
  J Appl Microbiol. 124(3):881-898
• Pubmed ID:
  29280543
• Pubmed Central ID:
  PMC9491513
• Document Type:
  Journal Article
• Funding:
  U54 OH008085/OH/NIOSH CDC HHSUnited States/ ; U54OH008085/ACL/ACL HHSUnited States/
• Volume:
  124
• Issue:
  3
• Download URL:
  https://stacks.cdc.gov/view/cdc/121540/cdc_121540_DS1.pdf
• File Type:
  [PDF - 1.36 MB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:392735956ffc2fdf16c2882a3129c461b68910b436a1e6d364af5ff573ea12d9