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CONUS manual for evaluating insecticide resistance in mosquitoes using the CDC bottle bioassay kit
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April 14, 2022
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Alternative Title:Continental US manual for evaluating insecticide resistance in mosquitoes using the CDC bottle bioassay kit
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Description:The primary objective is to provide guidance for using the CDC bottle bioassay as a surveillance tool for detecting insecticide resistance in mosquito vector populations. This document is intended for state and local public health officials and vector control specialists.
Insecticide resistance in a vector population is initially detected and characterized by using some sort of bioassay such as the World Health Organization larval or adult bioassay, topical assays, field cage tests, or the CDC bottle bioassay to determine whether a particular insecticide is effective against local mosquito species. Ideally, this essential question should be answered before a specific insecticide formulation is chosen and procured for vector control.
The Centers for Disease Control and Prevention (CDC) bottle bioassay is a surveillance tool for detecting insecticide resistance in vector populations. The assay is designed to help determine if the active ingredient in an insecticide formulation may be effective against a local mosquito population at a specific location at a given time. This information, combined with results of bioassays using enzyme inhibitors and those of biochemical and molecular assays, can assist in determining the resistant mechanism(s) present and guide decisions of which insecticide to use.
The aim of this document is to provide a practical laboratory manual that describes how to perform and interpret the CDC bottle bioassay for CONUS. Information for resistance testing can also be obtained from the CDC website at https://www.cdc.gov/zika/vector/insecticide-resistance.html.
CS330338-A
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CONUS-508.pdf
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