Evaluation of methods for detection of β-lactamase production in MSSA
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Evaluation of methods for detection of β-lactamase production in MSSA

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English
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Details:

  • Alternative Title:
    J Antimicrob Chemother
  • Personal Author:
  • Description:
    Objectives:

    Correct determination of penicillin susceptibility is pivotal for using penicillin in the treatment of Staphylococcus aureus infections. This study examines the performance of MIC determination, disc diffusion and a range of confirmatory tests for detection of penicillin susceptibility in S. aureus.

    Methods:

    A total of 286 consecutive penicillin-susceptible S. aureus blood culture isolates as well as a challenge set of 62 MSSA isolates were investigated for the presence of the blaZ gene by PCR and subjected to penicillin-susceptibility testing using broth microdilution MIC determination, disc diffusion including reading of the zone edge, two nitrocefin tests and the cloverleaf test.

    Results:

    Using PCR-based detection of blaZ as the gold standard, both broth microdilution MIC testing and disc diffusion testing resulted in a relatively low accuracy (82%–93%) with a sensitivity ranging from 49%–93%. Among the confirmatory tests, the cloverleaf test performed with 100% accuracy, while zone edge interpretation and nitrocefin-based tests increased the sensitivity of β-lactamase detection to 96%–98% and 82%–96% when using MIC determination or disc diffusion as primary test, respectively.

    Conclusions:

    This investigation showed that reliable and accurate detection of β-lactamase production in S. aureus can be obtained by MIC determination or penicillin disc diffusion followed by interpretation of the zone edge as a confirmatory test for apparently penicillin-susceptible isolates. The more cumbersome cloverleaf test can also be used. Nitrocefin-based tests should not be used as the only test for confirmation of a presumptive β-lactamase-negative isolate.

  • Subjects:
  • Source:
    J Antimicrob Chemother. 76(6):1487-1494
  • Pubmed ID:
    33615356
  • Pubmed Central ID:
    PMC9479532
  • Document Type:
    Journal Article
  • Volume:
    76
  • Issue:
    6
  • Collection(s):
  • Main Document Checksum:
  • Download URL:
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