Use of TaqMan Array Card for the Detection of Respiratory Viral Pathogens in Children under 5 Years Old Hospitalised with Acute Medical Illness in Ballabgarh, Haryana, India

Details

• Alternative Title:
  Indian J Med Microbiol
• Personal Author:
  Gaur, Bharti ; Saha, Siddhartha ; Iuliano, A. Danielle ; Rai, Sanjay K. ; Krishnan, Anand ; Jain, Seema ; Whitaker, Brett ; Winchell, Jonas ; Lal, Renu B. ; Broor, Shobha
• Description:
  Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1-3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%-100%) and specificity (98%-100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations.
• Subjects:
  Child, Preschool Humans India Infant Infant, Newborn Lab-On-A-Chip Devices Microfluidic Analytical Techniques Molecular Diagnostic Techniques Real-Time Polymerase Chain Reaction Respiratory Tract Infections Sensitivity And Specificity Viruses

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• Keywords:
  Micro-fluidic Card Sensitivity Specificity Viral Pathogen Diagnosis
• Source:
  Indian J Med Microbiol. 37(1):105-108
• Pubmed ID:
  31424019
• Pubmed Central ID:
  PMC9257431
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/ ; U01 IP000206/IP/NCIRD CDC HHSUnited States/
• Volume:
  37
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:df61eb3c41e6ac2409d26f3d009989ff16d60c78dfcde1197718a4ece2b6153c
• Download URL:
  https://stacks.cdc.gov/view/cdc/119352/cdc_119352_DS1.pdf
• File Type:
  [PDF - 167.39 KB ]