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Use of TaqMan Array Card for the Detection of Respiratory Viral Pathogens in Children under 5 Years Old Hospitalised with Acute Medical Illness in Ballabgarh, Haryana, India
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2019
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Source: Indian J Med Microbiol. 37(1):105-108
[PDF-167.39 KB]
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Alternative Title:Indian J Med Microbiol
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Description:Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1-3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%-100%) and specificity (98%-100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations.
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Pubmed ID:31424019
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Pubmed Central ID:PMC9257431
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Volume:37
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Issue:1
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