Retrospective Genomic Characterization of a 2017 Dengue Virus Outbreak, Burkina Faso

Letizia, Andrew G.; Pratt, Catherine B.; Wiley, Michael R.; Fox, Anne T.; Mosore, Mba; Agbodzi, Bright; Yeboah, Clara; Kumordjie, Selassie; Di Paola, Nicholas; Assana, Kone Cisse; Coulidiaty, David; Ouedraogo, Casimir; Bonney, Joseph H. Kofi; Ampofo, William; Tarnagda, Zékiba; Sangaré, Lassana

doi:10.3201/eid2806.212491

i

Retrospective Genomic Characterization of a 2017 Dengue Virus Outbreak, Burkina Faso

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6 2022
By Letizia, Andrew G. ; Pratt, Catherine B. ; Wiley, Michael R. ; ...

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English

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Alternative Title:

Emerg Infect Dis
Personal Author:

Letizia, Andrew G. ; Pratt, Catherine B. ; Wiley, Michael R. ; Fox, Anne T. ; Mosore, Mba ; Agbodzi, Bright ; Yeboah, Clara ; Kumordjie, Selassie ; Di Paola, Nicholas ; Assana, Kone Cisse ; Coulidiaty, David ; Ouedraogo, Casimir ; Bonney, Joseph H. Kofi ; Ampofo, William ; Tarnagda, Zékiba ; Sangaré, Lassana
Description:

Knowledge of contemporary genetic composition of dengue virus (DENV) in Africa is lacking. By using next-generation sequencing of samples from the 2017 DENV outbreak in Burkina Faso, we isolated 29 DENV genomes (5 serotype 1, 16 serotype 2 [DENV-2], and 8 serotype 3). Phylogenetic analysis demonstrated the endemic nature of DENV-2 in Burkina Faso. We noted discordant diagnostic results, probably related to genetic divergence between these genomes and the Trioplex PCR. Forward and reverse1 primers had a single mismatch when mapped to the DENV-2 genomes, probably explaining the insensitivity of the molecular test. Although we observed considerable homogeneity between the Dengvaxia and TetraVax-DV-TV003 vaccine strains as well as B cell epitopes compared with these genomes, we noted unique divergence. Continual surveillance of dengue virus in Africa is needed to clarify the ongoing novel evolutionary dynamics of circulating virus populations and support the development of effective diagnostic, therapeutic, and preventive countermeasures.
Subjects:

Dengue Dengue Virus Disease Outbreaks Genomics Humans Phylogeny Retrospective Studies Serogroup
Source:

Emerg Infect Dis. 2022; 28(6):1198-1210
Pubmed ID:

35608626
Pubmed Central ID:

PMC9155902
Document Type:

Journal Article
Place as Subject:

Burkina Faso
Volume:

28
Issue:

6
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:c853d6a0f6671cdb82dc8251563065e26c42193200fb5ac1e1001cfb8275b67c
Download URL:

https://stacks.cdc.gov/view/cdc/118183/cdc_118183_DS1.pdf
File Type:

[PDF - 8.51 MB ]

File Language:

English

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