Cadmium-induced ototoxicity in rat cochlear organotypic cultures

Details

• Alternative Title:
  Neurotox Res
• Personal Author:
  Liu, Hong ; Ding, Dalian ; Sun, Hong ; Jiang, Haiyan ; Wu, Xuewen ; Roth, Jerome A. ; Salvi, Richard
• Description:
  Cadmium (Cd), a widely used industrial metal, is extremely nephrotoxic and neurotoxic; however, its effects on the peripheral auditory system are poorly understood. To evaluate the ototoxicity of Cd, we treated cochlear organotypic cultures from postnatal day 3 rats with Cd concentrations from 10 to 500 μM for 24 or 48 h. Afterward, we evaluated the degree of damage to hair cells, auditory nerve fibers, and spiral ganglion neurons. Damage to the hair cells, auditory nerve fibers, and spiral ganglion neurons systematically increased in a dose and time-dependent manner. Exposure to Cd concentrations of 10 μM for 24 and 48 h resulted in minor inner and outer hair cell loss in the basal third of the cochlea. As Cd concentrations increased, toxicity spread toward the apex, also in a time-dependent manner. Treatment with 100 μM Cd for 48 h resulted in substantial (>30 %) hair cell loss over the entire cochlea. Cd was also toxic to auditory nerve fibers and spiral ganglion neurons; 100 μM of Cd for 24 h or 10 μM of Cd for 48 h resulted in considerable damage to auditory nerve fibers and spiral ganglion neurons. These findings are the first to demonstrate that Cd can cause significant lesions to peripheral auditory nerve fibers, spiral ganglion neurons, and sensory hair cells in organotypic cultures from postnatal cochleae.
• Subjects:
  Animals Apoptosis Article Cadmium Cochlea Cochlear Nerve Dose-Response Relationship, Drug Hair Cells Hair Cells, Auditory In Situ Nick-End Labeling Microscopy, Confocal Microscopy, Fluorescence Nerve Fibers Neurons Neurotoxins Rats, Sprague-Dawley Spiral Ganglion Spiral Ganglion Neurons Time Factors Tissue Culture Techniques

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• Source:
  Neurotox Res. 26(2):179-189
• Pubmed ID:
  24577639
• Pubmed Central ID:
  PMC9100876
• Document Type:
  Journal Article
• Funding:
  R01DC009219/DC/NIDCD NIH HHSUnited States/ ; R01 OH010235/OH/NIOSH CDC HHSUnited States/ ; R01 DC009091/DC/NIDCD NIH HHSUnited States/ ; R01DC009091/DC/NIDCD NIH HHSUnited States/ ; R01 DC009219/DC/NIDCD NIH HHSUnited States/
• Volume:
  26
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:6d9acbab9ff2858e9029f8fbdc04ba962188af53b2e847cd39b202b2252a5f45
• Download URL:
  https://stacks.cdc.gov/view/cdc/117330/cdc_117330_DS1.pdf
• File Type:
  [PDF - 2.72 MB ]