The Effects of Gamma Irradiation on Chemical Biomarker Recovery from Mixed Chemical/Biological Threat Exposure Specimens

Details

• Alternative Title:
  J Appl Lab Med
• Personal Author:
  Isenberg, Samantha L. ; Carter, Melissa D. ; Moon, Jonathan L. ; Laughlin, Sarah ; Petway, Marla ; Mojica, Mike A. ; Rood, Julia E. ; Gursky, Alexis K. ; Sheppard, Cody I. ; Bagarozzi, Dennis A. ; Pirkle, James L. ; Johnson, Rudolph C.
• Description:
  Background:
  
  Irradiative sterilization of clinical specimens prior to chemical laboratory testing provides a way to not only sterilize pathogens and ensure laboratorian safety but also preserve sample volume and maintain compatibility with quantitative chemical diagnostic protocols. Since the compatibility of clinical biomarkers with gamma irradiation is not well characterized, a subset of diagnostic biomarkers ranging in molecular size, concentration, and clinical matrix, was analyzed to determine recovery following gamma irradiation.
  
  Methods:
  
  Sample irradiation of previously characterized quality control materials (QCs) at 5 Mrad was carried out at the Gamma Cell Irradiation Facility at the Centers for Disease Control and Prevention (CDC) in Atlanta, GA. Following irradiation, the QCs were analyzed alongside non-irradiated QCs to determine analyte recovery between dosed and control samples.
  
  Results:
  
  Biomarkers for exposure to abrin, ricin, and organophosphorus nerve agents (OPNA) were analyzed for their stability following gamma irradiation. The diagnostic biomarkers included adducts to butyrylcholinesterase, abrine, and ricinine, respectively, and were recovered at over 90 % of their initial concentration.
  
  Conclusions:
  
  The results from this pilot study support the implementation of an irradiative sterilization protocol for possible mixed-exposure samples containing both chemical and biological threat agents (mixed CBTs). Furthermore, irradiative sterilization significantly reduces a laboratorian’s risk of infection from exposure to an infectious agent without compromising chemical diagnostic testing integrity, particularly for diagnostic assays in which the chemical analyte has been shown to be fully conserved following a 5 Mrad irradiative dose.
  
  
• Subjects:
  Alkaloids Article Biomarkers Biomarkers, Pharmacological Chemical Safety Chromatography, High Pressure Liquid Consumer Product Safety Equipment Safety Gamma Irradiation Gamma Rays Indole Alkaloids Irradiative Sterilization Laboratory Safety Mixed CBTs Mixed Chemical/biological Threats Pathogen Inactivation Pathogen Reduction Pilot Projects Pyridones Quality Control Radiation Dosage Sterilization

  More +
• Source:
  J Appl Lab Med. 5(2):273-280
• Pubmed ID:
  32445395
• Pubmed Central ID:
  PMC9022197
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  5
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:e38dbe1fbe98988a9dfd59ad9444874874a63656ae3270b56e07bab7c1a2fb95
• Download URL:
  https://stacks.cdc.gov/view/cdc/116644/cdc_116644_DS1.pdf
• File Type:
  [PDF - 488.89 KB ]