Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Heguy, Adriana ; Dimartino, Dacia ; Marier, Christian ; Zappile, Paul ; Guzman, Emily ; Duerr, Ralf ; Wang, Guiqing ; Plitnick, Jonathan ; Russell, Alexis ; Lamson, Daryl M. ; St. George, Kirsten
• Description:
  Of 379 severe acute respiratory syndrome coronavirus 2 samples collected in New York, USA, we detected 86 Omicron variant sequences containing Delta variant mutation P681R. Probable explanations were co-infection with 2 viruses or contamination/amplification artifact. Repeated library preparation with fewer cycles showed the P681R calls were artifactual. Unusual mutations should be interpreted with caution.
• Subjects:
  Artifacts Covid-19 Humans Mutation Sars-Cov-2
• Source:
  Emerg Infect Dis. 2022; 28(4):881-883
• Pubmed ID:
  35130474
• Pubmed Central ID:
  PMC8962901
• Document Type:
  Letter
• Place as Subject:
  New York
• Volume:
  28
• Issue:
  4
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:c8b5f17092bd92ade75fc9520430d87d729191d10333228deb8e67d64d3ac675
• Download URL:
  https://stacks.cdc.gov/view/cdc/116350/cdc_116350_DS1.pdf
• File Type:
  [PDF - 509.56 KB ]