Details:

Alternative Title:
Mol Genet Metab
Personal Author:
Dantonio, Paul D. ; Stevens, Germaine ; Hagar, Arthur ; Ludvigson, David ; Green, Daron ; ... More +
Dantonio, Paul D. ; Stevens, Germaine ; Hagar, Arthur ; Ludvigson, David ; Green, Daron ; Hannon, Harry ; Vogt, Robert F. Less -
Description:
A major factor in determining the suitability of a dried blood spot (DBS) specimen is the subjective nature of evaluation by laboratory personnel. Using newborn screening DBS specimen cards as they were submitted to a public health NBS program, we conducted a systematic pilot study of DBS evaluation by multiple experienced laboratory personnel (ELP) and by an automated optical scanning instrument (OSI) (CardScan (tm), BSD Robotics). OSI confirmed the satisfactory status of all newborn DBS specimen cards that passed initial review by the first ELP. Among the questionable cards selected for further review, 58% passed multiple ELP consensus assessment, and 62% passed OSI evaluation. The overall agreement between ELP and OSI was 86%. Among questionable specimen cards, ELP and OSI were more strongly correlated when multiple ELP assessment was unanimous. We conclude that subjective assessment by ELP is essential and that OSI evaluation is a useful adjunct when ELP assessment does not reach consensus. OSI further allows the selection of optimal locations for punching DBS from unsatisfactory or questionable specimens, optimizing the quality of interim analyses that may be conducted while repeat specimens are being collected. Instrument evaluation of specimen cards would also be valuable as an independent reference method for training laboratory and specimen collection personnel. OSI technology merits further studies to confirm and extend our findings.
Subjects:
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Algorithms Article Automation Dried Blood Spots Dried Blood Spot Testing Evaluation Humans Infant, Newborn Laboratory Personnel Neonatal Screening Newborn Bloodspot Screening Reproducibility Of Results Sensitivity And Specificity Specimen Quality Training
Source:
Mol Genet Metab. 113(1-2):62-66
Pubmed ID:
25095725
Pubmed Central ID:
PMC8944415
Document Type:
Journal Article
Funding:
CC999999/ImCDC/Intramural CDC HHSUnited States/
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:9155341dc684f3e807a1a193d9aa6701f1f9708b0f18419b48b2c45584868628
Download URL:
https://stacks.cdc.gov/view/cdc/115716/cdc_115716_DS1.pdf
File Type:

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