Persistence of SARS-Co-V-2 on N95 filtering facepiece respirators: implications for reuse
Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.
 
 
Help
Clear All

Persistence of SARS-Co-V-2 on N95 filtering facepiece respirators: implications for reuse



Public Access Version Available on: December 01, 2022, 12:00 AM
Please check back on the date listed above.
  • English

  • Details:

    • Alternative Title:
      J Occup Environ Hyg
    • Description:
      In response to the shortage of N95 filtering facepiece respirators for healthcare workers during the COVID-19 pandemic, the Centers for Disease Control and Prevention issued guidance for extended use and limited reuse of N95 FFRs to conserve supply. Previously worn N95 filtering facepiece respirators can serve as a source of pathogens, which can be transferred to the wearer while doffing and donning a respirator when practicing reuse. When practicing limited filtering facepiece respirators reuse, to reduce the risk of self-contamination, the Centers for Disease Control and Prevention recommends storing filtering facepiece respirators for five days between uses to allow for the decay of viable pathogens including SARS-CoV-2. This study assesses the persistence of the SARS-CoV-2 strain USA-WA1/2020 on N95 filtering facepiece respirators under controlled storage conditions for up to 5 days to inform the Centers for Disease Control and Prevention guidance. Coupons excised from six N95 filtering facepiece respirator models and glass slide coverslips were inoculated with the virus in a defined culture medium and in human saliva and stored at 20 °C and 20%, 45%, and 75% relative humidity. Statistically significant differences in SARS-CoV-2 half-lives were measured among the tested humidity levels with half-lives decreasing from an average of approximately 30 hr at 20% relative humidity to approximately 2 hr at 75% relative humidity. Significant differences in virus half-lives were also observed between the culture medium and saliva suspension media at 20% and 45% relative humidity with half lives up to 2.9 times greater when the virus was suspended in cell culture medium. The 5-day storage strategy, assessed in this study, resulted in a minimum of 93.4% reduction in viable virus for the most challenging condition (20% relative humidity, cell culture medium) and exceeding 99% reduction in virus at all other conditions.
    • Pubmed ID:
      34569911
    • Pubmed Central ID:
      PMC8915317
    • Document Type:
    • Collection(s):
    • Main Document Checksum:
    • File Type:
    • Supporting Files:
      No Additional Files

    More +

    You May Also Like

    Checkout today's featured content at