i
Dynamic 13C MR spectroscopy as an alternative to imaging for assessing cerebral metabolism using hyperpolarized pyruvate in humans
-
3 2022
-
-
Source: Magn Reson Med. 87(3):1136-1149
[PDF-2.08 MB]
Details:
-
Alternative Title:Magn Reson Med
-
Personal Author:
-
Description:Purpose:
This study is to investigate time-resolved 13C MR spectroscopy as an alternative to imaging for assessing pyruvate metabolism using hyperpolarized [1-13C]pyruvate in the human brain.
Methods:
Time-resolved 13C spectra were acquired from four axial brain slices of healthy human participants (n=4) after a bolus injection of hyperpolarized [1-13C]pyruvate. 13C MR spectroscopy with low flip-angle excitations and a multichannel 13C/1H dual-frequency RF coil were exploited for reliable and unperturbed assessment of hyperpolarized pyruvate metabolism. Slice-wise area under the curves (AUCs) of 13C-metabolites were measured and kinetic analysis was performed to estimate the production rates of lactate and HCO3–. Linear regression analysis between brain volumes and hyperpolarized signals was performed. Region-focused pyruvate metabolism was estimated using coil-wise 13C reconstruction. Reproducibility of hyperpolarized pyruvate exams was presented by performing two consecutive injections with a 45-min interval.
Results:
[1-13C]Lactate relative to the total 13C signal (tC) was 0.21–0.24 in all slices. [13C]HCO3–/tC was 0.065–0.091. Apparent conversion rate constants from pyruvate to lactate and HCO3– were calculated as 0.014–0.018s−1 and 0.0043–0.0056s−1, respectively. Pyruvate/tC and lactate/tC were in moderate linear relationships with fractional grey matter volume within each slice. White matter presented poor linear regression fit with hyperpolarized signals, and moderate correlations of the fractional cerebrospinal fluid volume with pyruvate/tC and lactate/tC were measured. Measured hyperpolarized signals were comparable between two consecutive exams with hyperpolarized [1-13C]pyruvate.
Conclusions:
Dynamic MRS in combination with multichannel RF coils is an affordable and reliable alternative to imaging methods in investigating cerebral metabolism using hyperpolarized [1-13C]pyruvate.
-
Subjects:
-
Source:
-
Pubmed ID:34687086
-
Pubmed Central ID:PMC8776582
-
Document Type:
-
Funding:
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
Supporting Files
-
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
xml 
docx 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg
