Use of partial N-gene sequences as a tool to monitor progress on rabies control and elimination efforts in Ethiopia

Details

• Alternative Title:
  Acta Trop
• Personal Author:
  Binkley, Laura ; Deressa, Asefa ; Shi, Mang ; Jara, Manuel ; Escobar, Luis E. ; Mauldin, Mathew R. ; Matheny, Audrey ; O’Quin, Jeanette ; Pieracci, Emily G. ; Kling, Chantal ; Hartloge, Claire ; Yimer, Getnet ; Abate, Ebba ; Gebreyes, Wondwossen ; Reynolds, Mary ; Belay, Ermias ; Shiferaw, Miriam ; Nakazawa, Yoshinori ; Velasco-Villa, Andres
  More +
• Description:
  Ethiopia is one of the African countries most affected by rabies. A coarse catalog of rabies viruses (RABV) was created as a benchmark to assess the impact of control and elimination activities. We evaluated a 726 bp amplicon at the end of the N-gene to infer viral lineages in circulation using maximum likelihood and Bayesian methods for phylogenetic reconstruction. We sequenced 228 brain samples from wild and domestic animals collected in five Ethiopian regions during 2010-2017. Results identified co-circulating RABV lineages that are causing recurrent spillover infections into wildlife and domestic animals. We found no evidence of importation of RABVs from other African countries or vaccine-induced cases in the area studied. A divergent RABV lineage might be involved in an independent rabies cycle in jackals. This investigation provides a feasible approach to assess rabies control and elimination efforts in resource-limited countries.
• Subjects:
  • Article
  • Dog rabies
  • Monitoring elimination
  • Co-circulating lineages
  • Resource-limited countries
  • Animals
  • Bayes Theorem
  • Ethiopia
  • Phylogeny
  • Rabies
  More +
• Source:
  Acta Trop. 221:106022
• Pubmed ID:
  34161816
• Pubmed Central ID:
  PMC8652542
• Document Type:
  Journal Article
• Funding:
  U2G GH001752/GH/CGH CDC HHSUnited States/
• Place as Subject:
  Ethiopia
• Volume:
  221
• Download URL:
  https://stacks.cdc.gov/view/cdc/112445/cdc_112445_DS1.pdf
• File Type:
  [PDF - 619.48 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:071b85cc058006c87373683d447054a9cf4ca0a3b5320bae7c02f5994eee6eb5