Use of real-time PCR as an alternative to conventional genotyping methods for the laboratory detection of lymphogranuloma venereum (LGV)

Details

• Alternative Title:
  Diagn Microbiol Infect Dis
• Personal Author:
  Woodson, Evonne N. ; Katz, Samantha S. ; Mosley, Sheree S. ; Danavall, Damien C. ; Bowden, Katherine E. ; Chi, Kai-Hua ; Raphael, Brian H.
• Description:
  Lymphogranuloma venereum (LGV) can be differentiated from non-LGV chlamydial infection using Sanger sequencing or molecular assays, including those that are commercially-available internationally. Here, we describe the performance of a rapid real-time PCR (RT-PCR)-based strategy in differentiating Chlamydia trachomatis infections associated with LGV or non-LGV serovars. One hundred three rectal swabs, previously genotyped using Sanger sequencing of the ompA gene as a reference method, were tested in the RT-PCR assays. All non-LGV specimens were correctly identified, but the RT-PCR failed to detect 1 LGV specimen, resulting in a sensitivity of 87.5% for the non-LGV/LGV RT-PCR assay. Additional performance characteristics (e.g., specificity, accuracy, and reproducibility) were all between 93% and 100% with a limit of detection ≤100 copies/reaction. Thus, this rapid RT-PCR method for LGV detection in clinical specimens is comparable to the reference method.
• Subjects:
  Bacterial Outer Membrane Proteins DNA, Bacterial Genome, Bacterial Genotype Humans Lymphogranuloma Venereum Molecular Diagnostic Techniques Real-Time Polymerase Chain Reaction Rectum Reproducibility Of Results Sensitivity And Specificity Sequence Analysis, DNA Serogroup

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• Source:
  Diagn Microbiol Infect Dis. 101(4):115532
• Pubmed ID:
  34571353
• Pubmed Central ID:
  PMC8650105
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  101
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:f2932e513e34267351fd7fd9ebe405cbfc53e97286b3e29f74126838245a9d27
• Download URL:
  https://stacks.cdc.gov/view/cdc/112441/cdc_112441_DS1.pdf
• File Type:
  [PDF - 545.59 KB ]