Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization

Details

• Alternative Title:
  Microb Genom
• Personal Author:
  Abd El Ghany, Moataz ; Barquist, Lars ; Clare, Simon ; Brandt, Cordelia ; Mayho, Matthew ; Joffre´, Enrique ; Sjöling, Åsa ; Turner, A. Keith ; Klena, John D. ; Kingsley, Robert A. ; Hill-Cawthorne, Grant A. ; Dougan, Gordon ; Pickard, Derek
• Description:
  Enterotoxigenic | (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing | in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.
• Subjects:
  CFA/I ETEC H10407 Research Articles RNAseq TraDIS Water Survival And Colonization
• Source:
  Microb Genom. 2021; 7(6)
• Pubmed ID:
  34110281
• Pubmed Central ID:
  PMC8461466
• Document Type:
  Journal Article
• Funding:
  WT_/Wellcome TrustUnited Kingdom/ ; BBS/E/F/000PR10348/BB_/Biotechnology and Biological Sciences Research CouncilUnited Kingdom/ ; BB/R012504/1/BB_/Biotechnology and Biological Sciences Research CouncilUnited Kingdom/
• Volume:
  7
• Issue:
  6
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:a445ea3847cd6e362fb208db45ad99894dee019b88001fcc30c045b4353ad627
• Download URL:
  https://stacks.cdc.gov/view/cdc/111657/cdc_111657_DS1.pdf
• File Type:
  [PDF - 2.28 MB ]